Cloning, characterization and functional analysis of an Alveoline-like protein in the shell of Pinctada fucata.
Cloning, characterization and functional analysis of an Alveoline-like protein in the shell of Pinctada fucata.
复制标题
珠母贝壳中类肺泡蛋白的克隆、表征和功能分析
DOI：
10.1038/s41598-018-29743-6
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发表时间：
2018-08-16
影响因子：
4.6
通讯作者：
Zhang R
中科院分区：
文献类型：
--
作者：
Kong J;Liu C;Wang T;Yang D;Yan Y;Chen Y;Liu Y;Huang J;Zheng G;Xie L;Zhang R
壳基质蛋白在生物矿化过程中具有重要作用。在过去的几十年里，SMPs的作用逐渐被揭示出来。2015年，本课题组在合浦珠母贝中鉴定出72种独特的SMPs，其中Alv蛋白在大珠母贝和珠母贝中有同源基因。本研究对Alv的全长cDNA序列进行了分析，并对Alv蛋白在壳形成过程中的功能进行了初步研究。对推导的蛋白质（Alv）进行了表征，其分子量为24.9 kDa，等电点为11.34，并进行了体内和体外功能分析。Alv基因在外套膜中有高表达，并能对切痕损伤产生应答。通过注射重组Alv（rAlv）抗体在体内抑制Alv蛋白的功能，破坏了棱柱结构，但促进了珍珠层的生长。Western blot和免疫荧光染色结果表明，天然Alv存在于棱柱层和珍珠层的EDTA不溶性基质中，并在棱柱层的内外侧具有不同的分布模式。总之，基质蛋白Alv的表征和功能分析可以扩展我们对基本基质蛋白及其在壳形成过程中的功能的理解。
Shell matrix proteins (SMPs) have important functions in biomineralization. In the past decades, the roles of SMPs were gradually revealed. In 2015, our group identified 72 unique SMPs in Pinctada fucata, among which Alveoline-like (Alv) protein was reported to have homologous genes in Pinctada maxima and Pinctada margaritifera. In this study, the full-length cDNA sequence of Alv and the functional analysis of Alv protein during shell formation were explored. The deduced protein (Alv), which has a molecular mass of 24.9 kDa and an isoelectric point of 11.34, was characterized, and the functional analyses was explored in vivo and in vitro. The Alv gene has high expression in mantle and could response to notching damage. The functional inhibition of Alv protein in vivo by injecting recombinant Alv (rAlv) antibodies destroyed prism structure but accelerated nacre growth. Western blot and immunofluorescence staining showed that native Alv exists in the EDTA-insoluble matrix of both prismatic and nacreous layers and has different distribution patterns in the inner or outer prismatic layer. Taken together, the characterization and functional analyses of matrix protein Alv could expand our understanding of basic matrix proteins and their functions during shell formation.
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影响因子：
3.8
作者：
Andersen, SO
通讯作者：
Andersen, SO
DOI：
10.1016/j.cbpb.2008.10.003
发表时间：
2009-02-01
影响因子：
2.2
作者：
Farre, B.;Dauphin, Y.
通讯作者：
Dauphin, Y.
影响因子：
64.8
作者：
ERBEN, HK;WATABE, N
通讯作者：
WATABE, N
影响因子：
4.3
作者：
Ichikawa, K;Shimomura, N;Ohkubo, N
通讯作者：
Ohkubo, N
影响因子：
3
作者：
Nudelman, F;Gotliv, BA;Weiner, S
通讯作者：
Weiner, S