Arrayed Imaging Reflectometry monitoring of anti-viral antibody production throughout vaccination and breakthrough Covid-19.
Arrayed Imaging Reflectometry monitoring of anti-viral antibody production throughout vaccination and breakthrough Covid-19.
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DOI：
10.1371/journal.pone.0277846
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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作者：
对COVID-19感染和疫苗接种的免疫反应是个体和多样的。需要了解针对当前和尚未发现的病毒突变的疫苗接种效力的时间轴。在对其他呼吸道病毒免疫的背景下评估对SARS-CoV-2的免疫也很有价值。在这里，我们证明了一个全自动的原型阵列成像反射系统进行可靠的纵向血清学对34重呼吸阵列的能力。该阵列包含呼吸道合胞病毒、季节性流感、常见人类冠状病毒、MERS、SARS-CoV-1和SARS-CoV-2的抗原。AIR测量由于血清抗体与阵列上的抗原结合而引起的反射率变化。从恢复期COVID-19供体和接种两剂mRNA疫苗方案的个体中收集样本。在首次接种前、首次接种后1周、第二次接种后1周以及此后每月采集一次疫苗接种样本。纳入了受试者子集的加强剂量和/或突破性感染后的信息。接种疫苗的个体的纵向样本表明SARS-CoV-2刺突抗体的上升和下降与适应性免疫应答的一般知识和其他研究一致。进行线性回归分析以了解与阵列上不同抗原结合的抗体之间的关系。我们的分析确定了密切相关的流感病毒株之间的强相关性，以及SARS-CoV-2，SARS-CoV-1和人类冠状病毒229 E之间的相关性。使用来自手指针刺的稀释全血的小测试提供了具有与血清相当的抗体结合的干净阵列。潜在的应用包括在暴露于多种呼吸道病毒的背景下评估免疫力，临床血清学，人口监测以促进公共卫生建议，以及针对新病毒和病毒突变的疫苗开发。
Immune responses to COVID-19 infection and vaccination are individual and varied. There is a need to understand the timeline of vaccination efficacy against current and yet to be discovered viral mutations. Assessing immunity to SARS-CoV-2 in the context of immunity to other respiratory viruses is also valuable. Here we demonstrate the capability of a fully automated prototype Arrayed Imaging Reflectometry system to perform reliable longitudinal serology against a 34-plex respiratory array. The array contains antigens for respiratory syncytial virus, seasonal influenza, common human coronaviruses, MERS, SARS-CoV-1, and SARS-CoV-2. AIR measures a change in reflectivity due to the binding of serum antibodies to the antigens on the array. Samples were collected from convalescent COVID-19 donors and individuals vaccinated with a two-dose mRNA vaccine regimen. Vaccinated samples were collected prior to the first dose, one week after the first dose, one week after the second dose, and monthly thereafter. Information following booster dose and/or breakthrough infection is included for a subset of subjects. Longitudinal samples of vaccinated individuals demonstrate a rise and fall of SARS-CoV-2 spike antibodies in agreement with general knowledge of the adaptive immune response and other studies. Linear Regression analysis was performed to understand the relationship between antibodies binding to different antigens on the array. Our analysis identified strong correlations between closely related influenza virus strains as well as correlations between SARS-CoV-2, SARS-CoV-1, and human coronavirus 229E. A small test of using diluted whole blood from a fingerstick provided clean arrays with antibody binding comparable to serum. Potential applications include assessing immunity in the context of exposure to multiple respiratory viruses, clinical serology, population monitoring to facilitate public health recommendations, and vaccine development against new viruses and virus mutations.
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影响因子：
15.1
作者：
Demonbreun AR;Sancilio A;Velez MP;Ryan DT;Saber R;Vaught LA;Reiser NL;Hsieh RR;D'Aquila RT;Mustanski B;McNally EM;McDade TW
通讯作者：
McDade TW
感染和疫苗接种后，在SARS-COV-2峰值蛋白上引起的交叉反应性抗体引起了保守的表位。
影响因子：
4.6
作者：
Geanes, Eric S.;LeMaster, Cas;Fraley, Elizabeth R.;Khanal, Santosh;McLennan, Rebecca;Grundberg, Elin;Selvarangan, Rangaraj;Bradley, Todd
通讯作者：
Bradley, Todd
DOI：
10.1126/science.abe8499
发表时间：
2020-12-18
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hou YJ;Chiba S;Halfmann P;Ehre C;Kuroda M;Dinnon KH 3rd;Leist SR;Schäfer A;Nakajima N;Takahashi K;Lee RE;Mascenik TM;Graham R;Edwards CE;Tse LV;Okuda K;Markmann AJ;Bartelt L;de Silva A;Margolis DM;Boucher RC;Randell SH;Suzuki T;Gralinski LE;Kawaoka Y;Baric RS
通讯作者：
Baric RS
DOI：
10.1056/nejmoa2201570
发表时间：
2022-05-05
期刊：
The New England journal of medicine
影响因子：
--
作者：
通讯作者：
--
DOI：
10.3390/pathogens11020186
发表时间：
2022-01-29
期刊：
Pathogens (Basel, Switzerland)
影响因子：
--
作者：
Embong AK;Nguyen-Contant P;Wang J;Kanagaiah P;Chaves FA;Fitzgerald TF;Zhou Q;Kosoy G;Branche AR;Miller BL;Zand MS;Sangster MY;Topham DJ
通讯作者：
Topham DJ