Raman spectral signature reflects transcriptomic features of antibiotic resistance in Escherichia coli.
Raman spectral signature reflects transcriptomic features of antibiotic resistance in Escherichia coli.
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DOI：
10.1038/s42003-018-0093-8
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发表时间：
2018
影响因子：
5.9
通讯作者：
Watanabe TM
中科院分区：
文献类型：
--
作者：
Germond A;Ichimura T;Horinouchi T;Fujita H;Furusawa C;Watanabe TM
能够从快速无标记显微观察中预测细菌的抗生素耐药性将有利于生物和生物医学领域的广泛应用。在这里，我们证明了无标记的拉曼光谱在监测的类型的电阻和获得性耐药的大肠杆菌的细菌种群的作用模式，在没有抗生素的效用。我们的发现是可重复的。此外，我们确定了最能预测作用模式的光谱区域，并探讨了拉曼特征是否与获得性抗性的遗传基础有关。光谱峰强度与一些有助于抗生素抗性基因的基因表达显著相关（假发现率，p < 0.05）。这些结果表明，抗生素耐药性的获得导致通过拉曼光谱特征和基因表达变化反映的广泛的代谢效应，暗示了这两层互补信息之间的可能关系。表征抗生素耐药性作用模式的技术对于开发新的抗微生物药物至关重要。Arno Germond等人已经使用拉曼光谱结合基因表达来研究细菌获得抗生素抗性时发生的大的代谢变化。
To be able to predict antibiotic resistance in bacteria from fast label-free microscopic observations would benefit a broad range of applications in the biological and biomedical fields. Here, we demonstrate the utility of label-free Raman spectroscopy in monitoring the type of resistance and the mode of action of acquired resistance in a bacterial population of Escherichia coli, in the absence of antibiotics. Our findings are reproducible. Moreover, we identified spectral regions that best predicted the modes of action and explored whether the Raman signatures could be linked to the genetic basis of acquired resistance. Spectral peak intensities significantly correlated (False Discovery Rate, p < 0.05) with the gene expression of some genes contributing to antibiotic resistance genes. These results suggest that the acquisition of antibiotic resistance leads to broad metabolic effects reflected through Raman spectral signatures and gene expression changes, hinting at a possible relation between these two layers of complementary information. Techniques for characterizing the mode of action of antibiotic resistance are crucial for developing new antimicrobial drugs. Arno Germond et al. have used Raman spectroscopy combined with gene expression to investigate large metabolic changes that occur when bacteria acquire antibiotic resistance.
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