Phospholipase Dδ and phosphatidic acid mediate heat-induced nuclear localization of glyceraldehyde-3-phosphate dehydrogenase in Arabidopsis.
Phospholipase Dδ and phosphatidic acid mediate heat-induced nuclear localization of glyceraldehyde-3-phosphate dehydrogenase in Arabidopsis.
复制标题
DOI：
10.1111/tpj.15981
复制
发表时间：
2022-11
期刊：
影响因子：
7.2
通讯作者：
Wang, Xuemin
中科院分区：
文献类型：
--
作者：
Kim, Sang-Chul;Yao, Shuaibing;Zhang, Qun;Wang, Xuemin
胞浆甘油醛-3-磷酸脱氢酶（GAPC）是一种糖酵解酶，但在压力诱导下会发生核转位。我们以前报道，在响应热胁迫，GAPC积累在细胞核中，调节转录和耐热性。在这里，我们展示了介导热诱导拟南芥胞质GAPC核转位的细胞和分子机制。拟南芥磷脂酶D（PLD）基因pldδ和pldα 1 pld δ敲除突变体热诱导GAPC核积累和植物耐热性降低，而pldα 1不降低。这些变化通过与活性PLDδ的遗传互补而恢复到野生型，但不与催化失活的PLDδ互补。W1WQ PLDδ. GAPC过表达增强了幼苗的耐热性和热诱导基因的表达，但这种效应在pldδ背景下被取消。热胁迫使野生型植物核内PLD产物磷脂酸（PA）水平升高，而pldδ植物核内则无此变化。脂质标记显示PA和GAPC的热诱导核共定位，这是受损的锌，抑制PA-GAPC的相互作用，并通过膜运输抑制剂布雷菲德菌素A（BFA）。锌或BFA处理降低GAPC核积累和幼苗耐热性。我们的数据表明PLDδ和PA在GAPC热诱导核转位中起关键作用。我们提出PLDδ产生的PA通过脂质-蛋白质相互作用介导这一过程，并且脂质介导作为细胞导管将细胞膜上的应激扰动与应对热胁迫的植物中的核功能联系起来。
Cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPC) is a glycolytic enzyme, but undergoes stress-induced nuclear translocation for moonlighting. We previously reported that in response to heat stress, GAPC accumulated in the nucleus to modulate transcription and thermotolerance. Here we show a cellular and molecular mechanism that mediates heat-induced nuclear translocation of cytosolic GAPC in Arabidopsis thaliana. Heat-induced GAPC nuclear accumulation and plant heat tolerance were reduced in Arabidopsis phospholipase D (PLD) knockout mutants of pldδ and pldα 1pldδ, but not of pldα 1. These changes were restored to wild type by genetic complementation with active PLDδ, but not with catalytically inactive PLDδ. W1WQ PLDδ. GAPC overexpression enhanced the seedling thermotolerance and the expression of heat-inducible genes, but this effect was abolished in the pldδ background. Heat stress elevated the levels of the PLD product phosphatidic acid (PA) in the nucleus in wild type, but not in pldδ plants. Lipid labeling demonstrated the heat-induced nuclear co-localization of PA and GAPC, which was impaired by zinc, which inhibited the PA–GAPC interaction, and by the membrane trafficking inhibitor brefeldin A (BFA). The GAPC nuclear accumulation and seedling thermotolerance were also decreased by treatment with zinc or BFA. Our data suggest that PLDδ and PA are critical for the heat-induced nuclear translocation of GAPC. We propose that PLDδ-produced PA mediates the process via lipid–protein interaction and that the lipid mediation acts as a cellular conduit linking stress perturbations at cell membranes to nuclear functions in plants coping with heat stress.
登录
查看更多内容
影响因子：
7.4
作者：
de Jong, Femke;Munnik, Teun
通讯作者：
Munnik, Teun
影响因子：
2.9
作者：
HARLOS, K;EIBL, H
通讯作者：
EIBL, H
影响因子：
--
作者：
Henkels, Karen M.;Miller, Taylor E.;Gomez-Cambronero, Julian
通讯作者：
Gomez-Cambronero, Julian
影响因子：
4.5
作者：
Henry E;Fung N;Liu J;Drakakaki G;Coaker G
通讯作者：
Coaker G
影响因子：
4.1
作者：
McLoughlin, Fionn;Arisz, Steven A.;Testerink, Christa
通讯作者：
Testerink, Christa