A phosphate starvation responsive malate dehydrogenase, GmMDH12 mediates malate synthesis and nodule size in soybean (Glycine max)
A phosphate starvation responsive malate dehydrogenase, GmMDH12 mediates malate synthesis and nodule size in soybean (Glycine max)
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GmMDH12 是一种磷酸盐饥饿响应型苹果酸脱氢酶，可介导大豆 (Glycine max) 中的苹果酸合成和根瘤大小
DOI：
10.1016/j.envexpbot.2021.104560
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发表时间：
2021-09
影响因子：
5.7
通讯作者：
Tian Jiang
中科院分区：
文献类型：
--
作者：
Zhu Shengnan;Chen Zhijian;Xie Baoxing;Guo Qi;Chen Minhui;Liang Cuiyue;Bai Zhenlong;Wang Xiurong;Wang Huicong;Liao Hong;Tian Jiang
在豆科植物根瘤中，苹果酸通常被认为为根瘤类菌呼吸和 NH4+ 同化骨架提供碳。尽管有人认为磷（P）缺乏会通过对碳分配产生不利影响来抑制豆科植物根瘤生长，但大豆（Glycine max）中苹果酸合成的分子机制及其在根瘤生长中的作用仍然很大程度上未知。在这项研究中，我们发现缺磷导致大豆根瘤大小（单个大豆根瘤的鲜重）显着下降，同时苹果酸浓度增加。同时，在大豆基因组中鉴定出 16 个苹果酸脱氢酶 (GmMDH) 成员，并研究了它们在大豆根瘤中响应磷缺乏的表达模式。其中，GmMDH12 因磷酸盐 (Pi) 饥饿而上调。 GmMDH12 的酶分析揭示了其在体外通过还原草酰乙酸形成苹果酸来催化苹果酸合成的功能。此外，GmMDH12的过表达导致转基因大豆根瘤中的根瘤尺寸显着减小，但苹果酸和几种碳水化合物的浓度显着增加。综上所述，这些结果表明，缺磷会由于苹果酸合成的增强而抑制大豆根瘤的大小，而苹果酸的合成受 GmMDH12 转录本的调节。
In legume nodules, malate is generally considered to provide carbon for both nodule bacteroid respiration and skeletons for NH4+assimilation. Although it has been suggested that phosphorus (P) deficiency inhibits legume nodule growth through adversely affecting carbon partitioning, the molecular mechanisms underlying malate synthesis and its roles in nodule growth remain largely unknown in soybean (Glycine max). In this study, we found that P deficiency led to a significant decrease of soybean nodule size (fresh weight of individual soybean nodule), accompanying with increased malate concentration. Meanwhile, 16 malate dehydrogenase (GmMDH) members were identified in the soybean genome, and their expression patterns were investigated in soybean nodules in responses to P deficiency. Among them,GmMDH12was up-regulated by phosphate (Pi) starvation. Enzymatic analysis of GmMDH12 revealed its functions in catalyzing malate synthesis through reduction of oxaloacetate to form malate in vitro. Furthermore, overexpression ofGmMDH12resulted in a significant decrease in nodule size, but a significant increase in concentrations of malate and several carbohydrates in transgenic soybean nodules. Taken together, these results suggest that P deficiency inhibits soybean nodule size due to enhancement of malate synthesis, which is regulated byGmMDH12transcripts.
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影响因子：
6.9
作者：
Sulieman S;Ha CV;Schulze J;Tran LS
通讯作者：
Tran LS
影响因子：
4.3
作者：
P. Vauclare;R. Bligny;E. Gout;Valentine De Meuron;François Widmer
通讯作者：
P. Vauclare;R. Bligny;E. Gout;Valentine De Meuron;François Widmer
影响因子：
7.4
作者：
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通讯作者：
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影响因子：
3.9
作者：
Ding, Y;Ma, QH
通讯作者：
Ma, QH
影响因子：
6.2
作者：
L. Qin;Meihuan Wang;Liyu Chen;Xuejiao Liang;Zhigeng Wu;Zhihao Lin;J. Zuo;Xiangyang Feng;Jing Zhao-;H. Liao;H. Ye
通讯作者：
L. Qin;Meihuan Wang;Liyu Chen;Xuejiao Liang;Zhigeng Wu;Zhihao Lin;J. Zuo;Xiangyang Feng;Jing Zhao-;H. Liao;H. Ye