Partial Sperm beta1 Integrin Subunit Deletion Proves its Involvement in Mouse Gamete Adhesion/Fusion.
Partial Sperm beta1 Integrin Subunit Deletion Proves its Involvement in Mouse Gamete Adhesion/Fusion.
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DOI：
10.3390/ijms21228494
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发表时间：
2020-11-11
影响因子：
5.6
通讯作者：
Ziyyat A
中科院分区：
文献类型：
--
作者：
Barraud-Lange V;Ialy-Radio C;Chalas C;Holtzmann I;Wolf JP;Barbaux S;Ziyyat A
我们之前已经使用抗体证明，精子α6beta1整合素参与了小鼠配子的体外融合。在这里，我们报告了精子Itgb1基因的条件性敲除。Itgb1基因缺失或不缺失导致精子融合能力严重丧失，精子聚集在体外受精卵母细胞的卵黄周间隙。这些数据表明，参与配子黏附/融合的是精子，而不是卵母细胞。奇怪的是，基因敲除的男性在体内能够生育，可能是因为Cre介导的精子Itgb1花基因的不完全缺失。事实上，这在Western印迹分析中得到了证实，并得到了通过将部分缺失的精子Itgb1的雄性与产生完全缺失的Itgb1卵母细胞的雌性交配而获得的幼崽的存活率和产仔数的证实。由于Itgb1缺失在小鼠围着床期的全部致命性，我们推测Itgb1切除后逃脱的精子似乎优先用于体内受精。在这里，我们首次展示了精子Itgb1基因的缺失，甚至部分缺失，会使精子无法正常受精。然而，为了阐明其在受精过程中作用的重要性的问题，有必要使用在雄性生殖细胞中更有效地表达重组酶的小鼠进行进一步的研究。
We have previously shown, using antibodies, that the sperm alpha6beta1 integrin is involved in mouse gamete fusion in vitro. Here we report the conditional knockdown of the sperm Itgb1 gene. It induced a drastic failure of sperm fusogenic ability with sperm accumulation in the perivitelline space of in vitro inseminated oocytes deleted or not for the Itgb1 gene. These data demonstrate that sperm, but not oocyte, beta1 integrin subunit is involved in gamete adhesion/fusion. Curiously, knockdown males were fertile in vivo probably because of the incomplete Cre-mediated deletion of the sperm Itgb1 floxed gene. Indeed, this was shown by Western blot analysis and confirmed by both the viability and litter size of pups obtained by mating partially sperm Itgb1 deleted males with females producing completely deleted Itgb1 oocytes. Because of the total peri-implantation lethality of Itgb1 deletion in mice, we assume that sperm that escaped the Itgb1 excision seemed to be preferentially used to fertilize in vivo. Here, we showed for the first time that the deletion, even partial, of the sperm Itgb1 gene makes the sperm unable to normally fertilize oocytes. However, to elucidate the question of the essentiality of its role during fertilization, further investigations using a mouse expressing a recombinase more effective in male germ cells are necessary.
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影响因子：
11.8
作者：
Hirota T;Blakeley P;Sangrithi MN;Mahadevaiah SK;Encheva V;Snijders AP;ElInati E;Ojarikre OA;de Rooij DG;Niakan KK;Turner JMA
通讯作者：
Turner JMA
影响因子：
6.1
作者：
Baillie, HS;Pacey, AA;Barratt, CLR
通讯作者：
Barratt, CLR
影响因子：
7.8
作者：
Miller, BJ;Georges-Labouesse, E;Myles, DG
通讯作者：
Myles, DG
影响因子：
2.8
作者：
Osycka-Salut, C. E.;Martinez-Leon, E.;Perez-Martinez, S.
通讯作者：
Perez-Martinez, S.
影响因子：
10.5
作者：
FASSLER, R;MEYER, M
通讯作者：
MEYER, M