Extrachromosomal circular DNA as a vehicle to gene transfer in plants.
Extrachromosomal circular DNA as a vehicle to gene transfer in plants.
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DOI：
10.1093/plphys/kiad380
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发表时间：
2023-08-31
期刊：
影响因子：
7.4
通讯作者：
Dunning, Luke T.
中科院分区：
文献类型：
--
作者：
Pereira, Lara;Dunning, Luke T.
适应环境变化对所有生物都是必要的，尤其是植物等固着生物。基因表达增加是核心应激反应之一，但从单个基因座转录的数量可能有限。解决这个问题的明显办法是复制所需的基因，但如何在一代人的时间内实现这一目标呢？通过染色体外环状DNA（eccDNA）的基因扩增是能够驱动该过程的基因组可塑性的一种形式（Peng et al. 2022）。这些环状分子在各种真核生物细胞中都有发现，它们的大小和遗传含量各不相同。在植物中，大多数eccDNA含有重复序列，基因间区域和基因片段，但很少找到完整的内源蛋白编码基因。一个众所周知的例外是最初在作物杂草Amaranthus palmeri中发现的400 kb eccDNA，它携带59个蛋白质编码基因，包括5-烯醇式莽草酸-3-磷酸合酶（EPSPS），除草剂草甘膦的靶标（Koo等人，2018）。值得注意的是，eccDNA分子上EPSPS基因拷贝数的增加赋予了除草剂抗性。先前通过荧光原位杂交（FISH）研究了EPSPS复制子的序列、结构、拷贝数和遗传形式（Koo et al. 2018）。EPSPS-FISH信号分析表明，该基因并没有整合到基因组中，而是以染色体外元件的形式存在，但它们在中期固定在染色体上。在体细胞中，有多达80个复制子拷贝，它们能够通过染色体系留传递给后代。杂交敏感和抗性A. Palmeri植物显示复制子被大多数后代遗传，
News Adaptation to environmental change is necessary in all organisms, especially in sessile organisms such as plants. Increased gene expression is one of the core stress responses, but there can be limits to how much can be transcribed from a single locus. The obvious solution to this problem is to duplicate the needed genes, but how can this be achieved within a generation? Gene amplification through extrachromosomal circular DNA (eccDNA) is one form of genome plasticity capable of driving this process (Peng et al. 2022). These circular molecules have been observed in all kinds of eukaryote cells, where they can vary in size and genetic content. In plants, most eccDNA contain repetitive sequences, intergenic regions, and genic fragments, but it is rare to find full endogenous protein-coding genes. A well-known exception is a 400-kb eccDNA initially found in the crop weed Amaranthus palmeri, which carries 59 protein-coding genes, including the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), target of the herbicide glyphosate (Koo et al. 2018). Remarkably, the increased gene copy number of EPSPS on the eccDNA molecules confers herbicide resistance.The sequence, structure, copy number, and form of inheritance of the EPSPS replicon were previously investigated through fluorescence in situ hybridization (FISH)(Koo et al. 2018). The EPSPS-FISH signals showed that this important herbicide resistance gene was not integrated in the genome but presented as extrachromosomal elements, yet they anchored to the chromosomes during metaphase. In somatic cells, there were up to 80 copies of the replicon, and they were able to be transmitted to the progeny via chromosome tethering. Crossing sensitive and resistant A. palmeri plants showed that the replicon was inherited by most offspring,
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影响因子：
4.5
作者：
Lanciano S;Carpentier MC;Llauro C;Jobet E;Robakowska-Hyzorek D;Lasserre E;Ghesquière A;Panaud O;Mirouze M
通讯作者：
Mirouze M
影响因子：
4.5
作者：
Demeke MM;Foulquié-Moreno MR;Dumortier F;Thevelein JM
通讯作者：
Thevelein JM
影响因子：
4.1
作者：
Nandula, Vijay K.;Wright, Alice A.;Molin, William T.
通讯作者：
Molin, William T.
DOI：
10.1073/pnas.1719354115
发表时间：
2018-03-27
影响因子：
11.1
作者：
Koo DH;Molin WT;Saski CA;Jiang J;Putta K;Jugulam M;Friebe B;Gill BS
通讯作者：
Gill BS