Selective pressure of antibiotics on ARGs and bacterial communities in manure-polluted freshwater-sediment microcosms.
Selective pressure of antibiotics on ARGs and bacterial communities in manure-polluted freshwater-sediment microcosms.
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抗生素对肥料污染的淡水污水缩略学的ARG和细菌群落的选择性压力。
DOI：
10.3389/fmicb.2015.00194
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发表时间：
2015
影响因子：
5.2
通讯作者：
Zeng Z
中科院分区：
文献类型：
--
作者：
Xiong W;Sun Y;Ding X;Wang M;Zeng Z
本研究旨在探讨抗生素对粪便污染水环境中抗生素耐药基因(ARGs)和细菌群落的选择压力。在淡水-沉积物微宇宙中设立三个处理组：四环素组、磺胺类药物组和氟喹诺酮类药物组。在处理后第14天采集沉积物和水样。分析了抗生素浓度、ARGs丰度和细菌群落组成。用超高效液相色谱-电喷雾串联质谱法测定抗生素浓度。用实时荧光定量聚合酶链式反应检测精氨酸蛋白的丰度。通过扩增片段测序分析细菌群落组成。在处理组分析的三类抗生素中，沉积物样品中积累量为四环素类；氟喹诺酮类磺胺类药物；水样中为磺胺类四环素类。在处理组中，沉积物样品中某些tet耐药基因的相对丰度[tet(W)和tet(X)]和质粒介导的喹诺酮类耐药(PMQR)基因[oqx(B)和aac(6‘)-Ib]的相对丰度显著高于配对水样。四环素类药物对沉积物样品中的革兰氏变态杆菌、梭状芽胞杆菌和沙门氏菌、大肠杆菌/志贺氏菌、梭状芽孢杆菌、营养单胞菌等细菌有显著的选择性。磺胺类和氟喹诺酮类对细菌群落也有明显的选择性。结果表明，底泥为细菌在抗生素污染下的抗性基因tet(W)和tet(X)以及PMQR基因[oqx(B)和aac(6‘)-Ib]的维持和持续提供了理想的环境。结果还强调，抗生素显著地选择了特定的细菌群落，包括与机会性病原体相关的分类群。
The aim of this study was to investigate selective pressure of antibiotics on antibiotic resistance genes (ARGs) and bacterial communities in manure-polluted aquatic environment. Three treatment groups were set up in freshwater-sediment microcosms: tetracyclines group, sulfonamides group and fluoroquinolones group. Sediment and water samples were collected on day 14 after treatment. Antibiotic concentrations, ARGs abundances and bacterial community composition were analyzed. Antibiotic concentrations were determined by ultra-performance liquid chromatography-electrospray tandem mass spectrometry. ARGs abundances were quantified by real time quantitative PCR. Bacterial community composition was analyzed based on amplicon sequencing. Of the three classes of antibiotics analyzed in the treatment groups, accumulation amounts were tetracyclines> fluoroquinolone> sulfonamides in the sediment samples, while they were sulfonamides> fluoroquinolone> tetracyclines in the water samples. In the treatment groups, the relative abundances of some tet resistance genes [tet(W) and tet(X)] and plasmid-mediated quinolone resistance (PMQR) genes [oqx(B) and aac(6′)-Ib] in sediment samples were significantly higher than those in the paired water samples. Tetracyclines significantly selected the bacterial classes including Gammaproteobacteria, Clostridia, and the genera including Salmonella, Escherichia/Shigella, Clostridium, Stenotrophomonas in sediment samples. The significant selection on bacterial communities posed by sulfonamides and fluoroquinolones was also observed. The results indicated that sediment may supply an ideal setting for maintenance and persistence of tet resistance genes [tet(W) and tet(X)] and PMQR genes [oqx(B) and aac(6′)-Ib] under antibiotic pollution. The results also highlighted that antibiotics significantly selected specific bacterial communities including the taxa associated with opportunistic pathogens.
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影响因子：
6.7
作者：
Gullberg E;Cao S;Berg OG;Ilbäck C;Sandegren L;Hughes D;Andersson DI
通讯作者：
Andersson DI
影响因子：
11.4
作者：
Arikan, Osman A.;Sikora, Lawrence J.;Foster, Gregory D.
通讯作者：
Foster, Gregory D.
影响因子：
13.6
作者：
Hsu, Jih-Tay;Chen, Chia-Yang;Ying, Chingwen
通讯作者：
Ying, Chingwen
影响因子：
4.4
作者：
Hong, Pei-Ying;Yannarell, Anthony C.;Mackie, Roderick I.
通讯作者：
Mackie, Roderick I.
影响因子：
11.4
作者：
Carstens, Keri L.;Gross, Aaron D.;Coats, Joel R.
通讯作者：
Coats, Joel R.