Temporal and Isoform-Specific Expression of CTBP2 Is Evolutionarily Conserved Between the Developing Chick and Human Retina.
Temporal and Isoform-Specific Expression of CTBP2 Is Evolutionarily Conserved Between the Developing Chick and Human Retina.
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DOI：
10.3389/fnmol.2021.773356
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发表时间：
2021
影响因子：
4.8
通讯作者：
Wahlin KJ
中科院分区：
文献类型：
--
作者：
Gage E;Agarwal D;Chenault C;Washington-Brown K;Szvetecz S;Jahan N;Wang Z;Jones MK;Zack DJ;Enke RA;Wahlin KJ
复杂的转录基因调控允许在视网膜发生过程中产生多方面的异构体，并且从单个位点转录的新型异构体可以具有无限的潜力来编码具有不同功能的多种蛋白质。在这项研究中，我们探讨了CTBP 2/RIBEYE基因位点和其独特的剧目的转录本是保守的脊椎动物。我们研究了转录辅助调节因子（CTBP 2）和带状突触特异性结构蛋白（RIBEYE）在鸡视网膜进行全面的组织化学和测序分析，以查明细胞和发育阶段特异性表达CTBP 2/RIBEYE在发育中的鸡视网膜。我们证明CTBP 2在视网膜发生早期开始在视网膜祖细胞中广泛表达，但在成熟视网膜中仅限于GABA能无长突细胞。因此，RIBEYE最初在祖细胞中表观遗传沉默，后来在感光细胞和双极细胞中表达，它们定位于带状突触。最后，我们使用多能干细胞衍生的视网膜类器官培养系统比较了CTBP 2/RIBEYE在发育中的人视网膜中的调节。这些分析表明，在鸡和人类视网膜发育过程中，CTBP 2/RIBEYE基因座的类似调节是由K50同源结构域转录因子家族的不同成员调节的。
Complex transcriptional gene regulation allows for multifaceted isoform production during retinogenesis, and novel isoforms transcribed from a single locus can have unlimited potential to code for diverse proteins with different functions. In this study, we explored the CTBP2/RIBEYE gene locus and its unique repertoire of transcripts that are conserved among vertebrates. We studied the transcriptional coregulator (CTBP2) and ribbon synapse-specific structural protein (RIBEYE) in the chicken retina by performing comprehensive histochemical and sequencing analyses to pinpoint cell and developmental stage-specific expression of CTBP2/RIBEYE in the developing chicken retina. We demonstrated that CTBP2 is widely expressed in retinal progenitors beginning in early retinogenesis but becomes limited to GABAergic amacrine cells in the mature retina. Inversely, RIBEYE is initially epigenetically silenced in progenitors and later expressed in photoreceptor and bipolar cells where they localize to ribbon synapses. Finally, we compared CTBP2/RIBEYE regulation in the developing human retina using a pluripotent stem cell derived retinal organoid culture system. These analyses demonstrate that similar regulation of the CTBP2/RIBEYE locus during chick and human retinal development is regulated by different members of the K50 homeodomain transcription factor family.
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影响因子：
6.2
作者：
He Y;He Z;Lin J;Chen C;Chen Y;Liu S
通讯作者：
Liu S
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
3.9
作者：
Anastasiadi D;Esteve-Codina A;Piferrer F
通讯作者：
Piferrer F
影响因子：
3.7
作者：
Kram YA;Mantey S;Corbo JC
通讯作者：
Corbo JC
影响因子：
9.8
作者：
Langouet-Astrie, Christophe J.;Meinsen, Annamarie L.;Enke, Raymond A.
通讯作者：
Enke, Raymond A.