Role of sodium in the RprY-dependent stress response in Porphyromonas gingivalis.
Role of sodium in the RprY-dependent stress response in Porphyromonas gingivalis.
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DOI：
10.1371/journal.pone.0063180
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Duncan MJ
中科院分区：
文献类型：
--
作者：
Krishnan K;Duncan MJ
牙龈卟啉单胞菌是一种革兰氏阴性口腔厌氧菌，与牙周病密切相关。牙龈沟内的环境变化会引发牙龈卟啉单胞菌的生长，并同时导致牙周健康向疾病的转变。细菌根据环境变化调整其生理机能，而双组分磷酸中继系统的基因调控是实现这种调整的机制之一。在牙龈卟啉单胞菌中，RprY 是一种孤儿反应调节因子，之前我们表明 RprY 调节子包括与氧化应激和钠代谢相关的基因。本研究的目标是识别诱导 rprY 的环境信号并阐明调节器在应激反应中的作用。在大肠杆菌中，RprY-LacZ 融合蛋白在贫钠培养基中被诱导，而牙龈卟啉单胞菌 rprY 突变体无法在类似的培养基中生长。通过多种方法，我们确定钠消耗会引起氧化应激相关基因的上调。此外，我们证明 RprY 直接与几种分子伴侣的启动子相互作用。此外，遗传和转录数据都表明该调节因子起到抑制因子的作用。我们得出结论，RprY 是控制牙龈卟啉单胞菌应激反应的调节因子之一，可能是通过充当阻遏蛋白，因为 rprY 突变体在贫钠培养基中表现出超应激反应，我们认为这会抑制生长。
Porphyromonas gingivalis is a Gram-negative oral anaerobe which is strongly associated with periodontal disease. Environmental changes in the gingival sulcus trigger the growth of P. gingivalis and a concurrent shift from periodontal health to disease. Bacteria adjust their physiology in response to environmental changes and gene regulation by two-component phospho-relay systems is one mechanism by which such adjustments are effected. In P. gingivalis RprY is an orphan response regulator and previously we showed that the RprY regulon included genes associated with oxidative stress and sodium metabolism. The goals of the present study were to identify environmental signals that induce rprY and clarify the role of the regulator in the stress response. In Escherichia coli an RprY-LacZ fusion protein was induced in sodium- depleted medium and a P. gingivalis rprY mutant was unable to grow in similar medium. By several approaches we established that sodium depletion induced up-regulation of genes involved in oxidative stress. In addition, we demonstrated that RprY interacted directly with the promoters of several molecular chaperones. Further, both genetic and transcription data suggest that the regulator acts as a repressor. We conclude that RprY is one of the regulators that controls stress responses in P. gingivalis, possibly by acting as a repressor since an rprY mutant showed a superstress reponse in sodium-depleted medium which we propose inhibited growth.
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