Removal of acenaphthene from water by Triton X-100-facilitated biochar-immobilized Pseudomonas aeruginosa.
Removal of acenaphthene from water by Triton X-100-facilitated biochar-immobilized Pseudomonas aeruginosa.
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DOI：
10.1039/c8ra03529f
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发表时间：
2018-06-21
期刊：
影响因子：
3.9
通讯作者：
He, Shanying
中科院分区：
文献类型：
--
作者：
Lu, Li;Li, Anan;Ji, Xueqin;Yang, Chunping;He, Shanying
通过添加非离子表面活性剂曲拉通X - 100，并以生物炭作为固定化载体，采用吸附法制备了曲拉通X - 100促进的生物炭固定化铜绿假单胞菌（TFBIP）材料，并将其用于处理水中的苊。结果表明，低浓度的曲拉通X - 100同时提高了生物炭的吸附能力和铜绿假单胞菌的降解活性，从而显著增强了固定化铜绿假单胞菌材料对水中苊的去除效果。与未添加曲拉通X - 100的对照组相比，低浓度的曲拉通X - 100使苊的去除率显著提高了20% - 50%。制备TFBIP的最佳条件为负载时间24小时，使用浓度为OD600 = 0.2的菌悬液，以及曲拉通X - 100浓度为10毫克/升。优化后的TFBIP材料在温度10 - 50℃、pH值4.5 - 10.5以及氯化钠浓度高达0.2摩尔/升的条件下，能够高效去除水中的苊。这种新型TFBIP材料可用于废水处理，也可直接用于受有机污染物污染土壤的修复。
以生物炭为固定化载体并添加非离子表面活性剂，制备出了一种新型高效的多环芳烃降解菌材料。
By adding the nonionic surfactant Triton X-100 and using biochar as an immobilization carrier, a Triton X-100-facilitated biochar-immobilized Pseudomonas aeruginosa (TFBIP) material was prepared using the sorption method and was used to treat acenaphthene in water. The results showed that a low concentration of Triton X-100 simultaneously promoted the sorption capacity of the biochar and the degradation activity of P. aeruginosa, thereby significantly enhancing the removal of acenaphthene from water by the immobilized P. aeruginosa material. Compared with the control without Triton X-100, a low concentration of Triton X-100 significantly increased the acenaphthene removal rate by 20–50%. The optimal conditions for preparing the TFBIP were a loading time of 24 h, the use of a bacterial suspension with a concentration of OD600 = 0.2, and a Triton X-100 concentration of 10 mg L−1. The optimized TFBIP material could efficiently remove acenaphthene from water at temperatures of 10–50 °C, pH values of 4.5–10.5, and NaCl concentrations of up to 0.2 mol L−1. The new TFBIP material can be used for the treatment of wastewater and may also be directly used for the remediation of soils contaminated with organic pollutants. A new efficient PAH-degrading bacterial material was produced by using biochar as an immobilization carrier and adding nonionic surfactant.
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