Cryptic Appearance of a New Clone of Vibrio cholerae Serogroup O1 Biotype El Tor in Calcutta, India
Cryptic Appearance of a New Clone of Vibrio cholerae Serogroup O1 Biotype El Tor in Calcutta, India
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印度加尔各答霍乱弧菌血清群 O1 生物型 El Tor 的新克隆隐秘出现
DOI：
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发表时间：
1997
影响因子：
2.6
通讯作者：
Y. Takeda
中科院分区：
文献类型：
--
作者：
S. Yamasaki;G. Nair;S. Bhattacharya;Shingo Yamamoto;H. Kurazono;Y. Takeda
在这项研究中，应用脉冲场凝胶电泳（PFGE）来确定在加尔各答被O139血清群暂时取代后重新出现的霍乱弧菌O1菌株是否与O139血清群出现之前分离的菌株不同。NotI消化在随机选择的24株代表不同时间范围的霍乱弧菌菌株中产生了总共11种不同的模式。在1993年7月以后分离的霍乱弧菌O1菌株中，观察到4种PFGE带型，命名为H至K，以H型为主。模式H明显不同于本研究中遇到的所有其他模式，包括1992年11月之前占优势的霍乱弧菌O 1 El Tor的模式A、B和C，以及占优势的霍乱弧菌O 139模式F。此外，H型也不同于目前在世界不同地区流行的霍乱弧菌O1 El Tor的4个致突变克隆群的代表菌株的NotI带型。霍乱弧菌O1新克隆的NotI片段未与O139特异性DNA探针杂交，表明霍乱弧菌O1新克隆中不存在O139遗传物质。使用O1特异性DNA探针的杂交数据再次区分了O139血清群形成之前存在的霍乱弧菌O1克隆和目前流行的O1菌株。
In this study, pulsed‐field gel electrophoresis (PFGE) was applied to determine if the Vibrio cholerae O1 strains which reappeared after being temporarily displaced in Calcutta by the O139 serogroup were different from those isolated before the advent of the O139 serogroup. NotI digestion generated a total of 11 different patterns among the 24 strains of V. cholerae randomly selected to represent different time frames. Among the V. cholerae O1 strains isolated after July 1993, 4 PFGE banding patterns designated as H through K were observed with pattern H dominating. Pattern H was distinctly different from all other patterns encountered in this study including patterns A, B and C of V. cholerae O1 El Tor, which dominated before November 1992, and pattern F, which was the dominant V. cholerae O139 pattern. Further, pattern H was also different from the NotI banding patterns of the representative strains of the 4 toxigenic clonal groups of V. cholerae O1 El Tor currently prevailing in different parts of the world. NotI fragments of the new clone of V. cholerae O1 did not hybridize with an O139 specific DNA probe, indicating that there was no O139 genetic material in the new clone of V. cholerae O1. Hybridization data with an O1‐specific DNA probe again differentiated between the clones of V. cholerae O1 existing before the genesis of the O139 serogroup and the O1 strains currently prevalent.
通过 DNA 菌落杂交检测产肠毒素大肠杆菌。
DOI：
10.1093/infdis/142.6.892
发表时间：
1980
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Moseley,SL;Huq,I;Alim,AR;So,M;Samadpour-Motalebi,M;Falkow,S
通讯作者：
Falkow,S