Assessment of unilateral ureter obstruction with multi-parametric MRI.
Assessment of unilateral ureter obstruction with multi-parametric MRI.
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DOI：
10.1002/mrm.26849
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发表时间：
2018-04
影响因子：
3.3
通讯作者：
Gore JC
中科院分区：
文献类型：
--
作者：
Wang F;Takahashi K;Li H;Zu Z;Li K;Xu J;Harris RC;Takahashi T;Gore JC
定量、多参数MRI（mpMRI）方法可以灵敏、客观地评估肾损伤和功能。本研究旨在评价一系列利用内源性对比度的MRI方法，包括弛豫率、来自定量磁化转移（qMT）的池大小比（PSR）、化学交换饱和转移（CEST）、核Overhauser增强（NOE）和表观扩散系数（ADC），在单侧输尿管梗阻（UUO）小鼠模型中检测与肾脏疾病相关的异常特征的灵敏度和特异性。在7 T下在UUO后1、3或6天对麻醉的C57 BL/6 N小鼠进行MRI扫描。石蜡组织切片在MRI后用Masson三色染色。与对侧肾相比，UUO肾皮质的舒张率R1和R2、PSR、NOE和ADC均降低。在UUO肾脏的皮质区未观察到CEST效应的显著变化。肾皮质的MRI参数变化与肾小管细胞死亡、肾小管萎缩、肾小管扩张、尿潴留和UUO肾皮质间质纤维化有关。多个MRI参数的测量提供了关于UUO产生的分子和细胞变化的综合信息。
Quantitative, multi-parametric MRI (mpMRI) methods may allow the assessment of renal injury and function in a sensitive and objective manner. This study aimed to evaluate an array of MRI methods that exploit endogenous contrasts including relaxation rates, pool size ratio (PSR) derived from quantitative magnetization transfer (qMT), chemical exchange saturation transfer (CEST), nuclear Overhauser enhancement (NOE) and apparent diffusion coefficient (ADC), for their sensitivity and specificity in detecting abnormal features associated with kidney disease in a murine model of unilateral ureter obstruction (UUO). MRI scans were performed in anesthetized C57BL/6N mice 1, 3 or 6 days after UUO at 7T. Paraffin tissue sections were stained with Masson trichrome following MRI. Compared to contralateral kidneys, the cortices of UUO kidneys showed decreases of relaxation rates R1 and R2, PSR, NOE and ADC. No significant changes in CEST effects were observed for the cortical region of UUO kidneys. The MRI parametric changes in renal cortex are related to tubular cell death, tubular atrophy, tubular dilation, and urine retention, and interstitial fibrosis in the cortex of UUO kidneys. Measurements of multiple MRI parameters provide comprehensive information about the molecular and cellular changes produced by UUO.
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