Sequential accumulation of dynein and its regulatory proteins at the spindle region in the Caenorhabditis elegans embryo.
Sequential accumulation of dynein and its regulatory proteins at the spindle region in the Caenorhabditis elegans embryo.
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秀丽隐杆线虫胚胎纺锤体区域动力蛋白及其调节蛋白的顺序积累。
DOI：
10.1038/s41598-022-15042-8
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发表时间：
2022-07-11
影响因子：
4.6
通讯作者：
Kimura, Akatsuki
中科院分区：
文献类型：
--
作者：
Torisawa, Takayuki;Kimura, Akatsuki
细胞质动力蛋白负责细胞周期中的各种细胞过程。其活性在细胞内空间和暂时调节的机制仍然难以捉摸。动力蛋白有多种调节蛋白，包括dynactin、NDEL 1/NUD-2和LIS 1。表征调节蛋白在体内的时空定位将有助于理解动力蛋白的细胞调节。在这里，我们专注于纺锤体的形成在秀丽隐杆线虫早期胚胎，其中动力蛋白及其调节蛋白易位从细胞质的纺锤体区域核膜破裂（NEBD）。我们发现，（i）一组有限的动力蛋白调控蛋白积累在纺锤体区域，（ii）的空间定位模式是不同的监管机构，和（iii）的监管蛋白不积累在纺锤体区域同时，但顺序。此外，NUD-2的积累在调节剂中是独特的。NUD-2在NEBD之前开始积累（pre-NEBD积累），并且与细胞质浓度相比表现出最高的富集。使用蛋白质注射方法，我们发现NUD-2的C-末端螺旋负责前NEBD积累。这些研究结果表明，一个很好的时间控制调节蛋白的亚细胞定位。
Cytoplasmic dynein is responsible for various cellular processes during the cell cycle. The mechanism by which its activity is regulated spatially and temporarily inside the cell remains elusive. There are various regulatory proteins of dynein, including dynactin, NDEL1/NUD-2, and LIS1. Characterizing the spatiotemporal localization of regulatory proteins in vivo will aid understanding of the cellular regulation of dynein. Here, we focused on spindle formation in the Caenorhabditis elegans early embryo, wherein dynein and its regulatory proteins translocated from the cytoplasm to the spindle region upon nuclear envelope breakdown (NEBD). We found that (i) a limited set of dynein regulatory proteins accumulated in the spindle region, (ii) the spatial localization patterns were distinct among the regulators, and (iii) the regulatory proteins did not accumulate in the spindle region simultaneously but sequentially. Furthermore, the accumulation of NUD-2 was unique among the regulators. NUD-2 started to accumulate before NEBD (pre-NEBD accumulation), and exhibited the highest enrichment compared to the cytoplasmic concentration. Using a protein injection approach, we revealed that the C-terminal helix of NUD-2 was responsible for pre-NEBD accumulation. These findings suggest a fine temporal control of the subcellular localization of regulatory proteins.
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影响因子：
7.5
作者：
Makarova, Maria;Oliferenko, Snezhana
通讯作者：
Oliferenko, Snezhana
影响因子：
3.3
作者：
Heppert, Jennifer K.;Pani, Ariel M.;Goldstein, Bob
通讯作者：
Goldstein, Bob
DOI：
10.1016/j.bbrc.2008.10.120
发表时间：
2008-12-26
影响因子：
3.1
作者：
Bradshaw, Nicholas J.;Ogawa, Fumiaki;Millar, J. Kirsty
通讯作者：
Millar, J. Kirsty
DOI：
10.1038/nrm2804
发表时间：
2009-12
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
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影响因子：
4
作者：
Cockell, MM;Baumer, M;Gönczy, P
通讯作者：
Gönczy, P