The nuclear GUCT domain-containing DEAD-box RNA helicases govern gametophytic and sporophytic development in Physcomitrium patens.
The nuclear GUCT domain-containing DEAD-box RNA helicases govern gametophytic and sporophytic development in Physcomitrium patens.
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含核闸门的死盒RNA解旋酶在Phandcomitrium patens中控制着配子型和孢子型发育。
DOI：
10.1007/s11103-021-01152-w
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发表时间：
2021-11
影响因子：
5.1
通讯作者：
Johansen W
中科院分区：
文献类型：
--
作者：
Perroud PF;Demko V;Ako AE;Khanal R;Bokor B;Pavlovič A;Jásik J;Johansen W
PpRH1/PpRH2是定位于细胞核的含有谷氨酸转化酶结构域的DEAD-BOX RNA解旋酶。它们与苔藓生命周期各个阶段的细胞和组织发育有关。含有dead -box的RNA解旋酶家族包含了一大批功能重要的酶，这些酶参与了RNA代谢的细胞过程，包括转录、加工、运输、翻译和衰变。研究表明，该蛋白家族在植物的营养和生殖发育过程以及对寒冷和高盐度等环境胁迫的响应中起着潜在的作用。我们在此重点研究了藓类Physcomitrium patens中含有GUCT结构域的RNA解旋酶的一个小的保守亚群。系统发育分析表明，含有GUCT结构域的RNA解旋酶在整个绿色谱系中形成了一个独特的保守分支。在该分支中，patens基因组具有两个密切相关的旁系RNA解旋酶，PpRH1和PpRH2被预测为核。利用基因荧光标记，我们发现PpRH1定位于原丝体的细胞核。PpRH1和PpRH2缺失的分析，无论是单独的还是共同的，都表明了它们在植物原丝体、配子体和孢子体细胞和组织发育中的潜在作用。此外，Δrh2和Δrh1/2叶根叶绿体的超微结构分析显示，在标准生长条件下，淀粉颗粒的积累与光合活性参数的变化有关。我们没有检测到温度或胁迫对原丝体生长或PpRH1和PpRH2表达的影响。综上所述，这些结果表明，含有谷氨酰胺的核RNA解旋酶在苔藓光合作用的直接或间接相关的发育过程中起主要作用。在线版本包含补充资料，网址为10.1007/s11103-021-01152-w。
In Physcomitrium patens, PpRH1/PpRH2 are GUCT-domain-containing DEAD-BOX RNA helicases localize to the nucleus. They are implicated in cell and tissue development in all stages of the moss life cycle. The DEAD-box-containing RNA helicase family encompasses a large and functionally important group of enzymes involved in cellular processes committed to the metabolism of RNA, including its transcription, processing, transport, translation and decay. Studies indicate this protein family has implied roles in plant vegetative and reproductive developmental processes as well as response to environmental stresses such has cold and high salinity. We focus here on a small conserved sub-group of GUCT domain-containing RNA helicase in the moss Physcomitrium patens. Phylogenetic analysis shows that RNA helicases containing the GUCT domain form a distinct conserved clade across the green lineage. In this clade, the P. patens genome possesses two closely related paralogues RNA helicases predicted to be nuclear, PpRH1 and PpRH2. Using in-locus gene fluorescent tagging we show that PpRH1 is localized to the nucleus in protonema. Analysis of PpRH1 and PpRH2 deletions, individually and together, indicates their potential roles in protonema, gametophore and sporophyte cellular and tissue development in P. patens. Additionally, the ultrastructural analysis of phyllid chloroplasts in Δrh2 and Δrh1/2 shows distinct starch granule accumulation under standard growth conditions associated with changes in photosynthetic activity parameters. We could not detect effects of either temperature or stress on protonema growth or PpRH1 and PpRH2 expression. Together, these results suggest that nuclear GUCT-containing RNA helicases play a role primarily in developmental processes directly or indirectly linked to photosynthesis activity in the moss P. patens. The online version contains supplementary material available at 10.1007/s11103-021-01152-w.
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影响因子：
9.2
作者：
Andersen, JS;Lyon, CE;Lamond, AI
通讯作者：
Lamond, AI
DOI：
10.1111/tpj.13062
发表时间：
2015-12
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Bush MS;Crowe N;Zheng T;Doonan JH
通讯作者：
Doonan JH
影响因子：
5.3
作者：
Lorkovic, ZJ;Herrmann, RG;Oelmuller, R
通讯作者：
Oelmuller, R
影响因子：
3.8
作者：
Kim, Myung-Hee;Sonoda, Yutaka;Imai, Ryozo
通讯作者：
Imai, Ryozo
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM