Indirect detection of labile solute proton spectra via the water signal using frequency-labeled exchange (FLEX) transfer.
Indirect detection of labile solute proton spectra via the water signal using frequency-labeled exchange (FLEX) transfer.
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DOI：
10.1021/ja909001q
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发表时间：
2010-02-17
影响因子：
15
通讯作者：
Van Zijl, Peter C. M.
中科院分区：
文献类型：
--
作者：
Friedman, Joshua I.;McMahon, Michael T.;Stivers, James T.;Van Zijl, Peter C. M.
我们报告了一种“交换率过滤”的磁共振方法，该方法允许在不干扰非交换质子的情况下检测低浓度溶液的可交换质子。相对于标准光谱，将化学移位特异性和提高灵敏度增加了数量级。 （k> 300s-1）在所谓的化学交换安全转移（CEST）MRI对比剂中，有望为研究核酸和蛋白质的高度动态区域提供一种实用的方法在封闭的无法访问状态和发生这种替代类型的标签和检测可交换质子的裸露状态之间，迅速移动。使用频率转移而不是饱和传递检测。
We report an “exchange-rate-filtered” magnetic resonance approach that allows the detection of exchangeable protons of low concentration solutes without interference of non-exchanging protons. This indirect detection of signals of multiple rapidly exchanging protons through the water signal can be achieved while retaining chemical shift specificity and increasing sensitivity by several orders of magnitude with respect to standard spectroscopy. This frequency labeled exchange (FLEX) transfer principle is applied to detect previously “invisible” protons of some nucleic acids, and peptides, as well as rapidly exchanging protons (k > 300s−1) in so-called chemical exchange saturation transfer (CEST) MRI contrast agents. FLEX methodology is expected to provide a practical approach for the study of highly dynamic regions of nucleic acids and proteins where amide, amino and imino groups are rapidly moving between a closed solvent inaccessible state and an exposed state where exchange occurs. This alternative type of labeling and detecting exchangeable protons is also expected to greatly benefit the development of new exchange-based MRI contrast agents, providing a method for multi-frequency detection using frequency transfer instead of saturation transfer.
影响因子：
3.3
作者：
McMahon, Michael T.;Gilad, Assaf A.;DeLiso, Marco A.;Berman, Stacey D. Cromer;Bulte, Jeff W. M.;van Zijl, Peter C. M.
通讯作者：
van Zijl, Peter C. M.
影响因子：
2.2
作者：
Ward, KM;Aletras, AH;Balaban, RS
通讯作者：
Balaban, RS