Genetic mapping and marker development for resistance of wheat against the root lesion nematode Pratylenchus neglectus.
Genetic mapping and marker development for resistance of wheat against the root lesion nematode Pratylenchus neglectus.
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DOI：
10.1186/1471-2229-13-230
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发表时间：
2013-12-31
影响因子：
5.3
通讯作者：
Mather DE
中科院分区：
文献类型：
--
作者：
Jayatilake DV;Tucker EJ;Bariana H;Kuchel H;Edwards J;McKay AC;Chalmers K;Mather DE
Rlnn 1基因位于小麦（Triticum aestivum L.）赋予对根病线虫Pratylenchus neglectus的中等抗性。在本研究之前，Rlnn 1与7A染色体上其他位点的连锁关系尚不清楚，也没有简单的共显性标记可用于小麦育种中Rlnn 1的选择。本研究的目的是（1）建立小麦7A染色体Rlnn 1区域的遗传图谱;（2）开发可用于分子标记辅助选择的分子标记，以提高小麦对P. neglectus的抗性。利用Excalibur/Kukri双单倍体群体定位了一个抗稻纵卷叶螟的大效应数量性状位点。该QTL与抗锈基因Lr 20/Sr 15、八氢番茄红素合成酶基因Psy-A1和10个分子标记（包括5个利用小麦-水稻比较基因组学和小麦表达序列标签设计的新标记）的位置一致。其中两个新标记适合用作分子诊断工具，以区分携带Rlnn 1和Lr 20/Sr 15的植物与不携带这些抗性基因的植物。Rlnn 1的基因组位置被证实位于染色体7A长臂的末端区域。分子标记的发展，提供了简单的替代昂贵的表型评估对P. neglectus在小麦育种的抗性。在王者之剑中，基因重组似乎在Rlnn 1区域被完全抑制。
The Rlnn1 locus, which resides on chromosome 7A of bread wheat (Triticum aestivum L.) confers moderate resistance against the root lesion nematode Pratylenchus neglectus. Prior to this research, the exact linkage relationships of Rlnn1 with other loci on chromosome 7A were not clear and there were no simple codominant markers available for selection of Rlnn1 in wheat breeding. The objectives of the research reported here were to (1) develop an improved genetic map of the Rlnn1 region of chromosome 7A and (2) develop molecular markers that could be used in marker-assisted selection to improve resistance of wheat against P. neglectus. A large-effect quantitative trait locus (QTL) for resistance against P. neglectus was genetically mapped using a population of Excalibur/Kukri doubled haploid lines. This QTL coincides in position with the rust resistance gene(s) Lr20/Sr15, the phytoene synthase gene Psy-A1 and 10 molecular markers, including five new markers designed using wheat-rice comparative genomics and wheat expressed sequence tags. Two of the new markers are suitable for use as molecular diagnostic tools to distinguish plants that carry Rlnn1 and Lr20/Sr15 from those that do not carry these resistance genes. The genomic location of Rlnn1 was confirmed to be in the terminal region of the long arm of chromosome 7A. Molecular markers were developed that provide simple alternatives to costly phenotypic assessment of resistance against P. neglectus in wheat breeding. In Excalibur, genetic recombination seems to be completely suppressed in the Rlnn1 region.
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影响因子：
4.4
作者：
Akhunov ED;Akhunova AR;Anderson OD;Anderson JA;Blake N;Clegg MT;Coleman-Derr D;Conley EJ;Crossman CC;Deal KR;Dubcovsky J;Gill BS;Gu YQ;Hadam J;Heo H;Huo N;Lazo GR;Luo MC;Ma YQ;Matthews DE;McGuire PE;Morrell PL;Qualset CO;Renfro J;Tabanao D;Talbert LE;Tian C;Toleno DM;Warburton ML;You FM;Zhang W;Dvorak J
通讯作者：
Dvorak J
影响因子：
2.9
作者：
Howitt, Crispin A.;Cavanagh, Colin R.;Pogson, Barry J.
通讯作者：
Pogson, Barry J.
影响因子：
56.9
作者：
Krattinger, Simon G.;Lagudah, Evans S.;Keller, Beat
通讯作者：
Keller, Beat
影响因子：
2.5
作者：
Manly, KF;Cudmore, RH;Meer, JM
通讯作者：
Meer, JM
DOI：
10.1186/1939-8433-6-4
发表时间：
2013-02-06
期刊：
Rice (New York, N.Y.)
影响因子：
--
作者：
Kawahara Y;de la Bastide M;Hamilton JP;Kanamori H;McCombie WR;Ouyang S;Schwartz DC;Tanaka T;Wu J;Zhou S;Childs KL;Davidson RM;Lin H;Quesada-Ocampo L;Vaillancourt B;Sakai H;Lee SS;Kim J;Numa H;Itoh T;Buell CR;Matsumoto T
通讯作者：
Matsumoto T