Sequenced dermatophyte strains: growth rate, conidiation, drug susceptibilities, and virulence in an invertebrate model.
Sequenced dermatophyte strains: growth rate, conidiation, drug susceptibilities, and virulence in an invertebrate model.
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DOI：
10.1016/j.fgb.2010.11.010
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发表时间：
2011-03
影响因子：
3
通讯作者：
White, Theodore C.
中科院分区：
文献类型：
--
作者：
Achterman, Rebecca R.;Smith, Adam R.;Oliver, Brian G.;White, Theodore C.
尽管皮肤真菌是世界上最常见的真菌感染原因，但人们对它们的基本生物学还不太了解。最近对五种代表性皮肤真菌物种的基因组进行了测序和注释，从而可以建立假说，说明它们是如何引起疾病的，并已适应了它们不同的环境。对这些菌株的微生物学的了解将是检验这些假说的关键。这项研究是第一次对这五种已测序的皮肤真菌菌株的微生物学特征进行总体描述。测定了不同菌种间的生长速度，其中石膏小孢子菌CBS118893生长最快，红色毛霉菌CBS118892生长最慢。我们还比较了不同的培养基产分生孢子的情况，发现当皮肤真菌在垫状琼脂上生长时，产孢量最高。我们测定了9种抗真菌药物的最低抑菌浓度(MIC)，并确认了对常用抗真菌药物的敏感性。最后，我们测试了毒力在小菜蛾(蜡蛾)幼虫模型，但发现结果变量。这些结果增加了我们对这些皮肤真菌菌株的微生物学和分子生物学的了解，并将有助于推进关于皮肤真菌的假设驱动的研究。
Although dermatophytes are the most common cause of fungal infections in the world, their basic biology is not well understood. The recent sequencing and annotation of the genomes of five representative dermatophyte species allows for the creation of hypotheses as to how they cause disease and have adapted to their distinct environments. An understanding of the microbiology of these strains will be essential for testing these hypotheses. This study is the first to generally characterize these five sequenced strains of dermatophytes for their microbiological aspects. We measured the growth rate on solid medium and found differences between species, with Microsporum gypseum CBS118893 having the fastest growth and Trichophyton rubrum CBS118892 the slowest. We also compared different media for conidia production and found that the highest numbers of conidia were produced when dermatophytes were grown on MAT agar. We determined the Minimum Inhibitory Concentration (MIC) of nine antifungal agents and confirmed susceptibility to antifungals commonly used as selectable markers. Finally, we tested virulence in the Galleria mellonella (wax moth) larvae model but found the results variable. These results increase our understanding of the microbiology and molecular biology of these dermatophyte strains and will be of use in advancing hypothesis-driven research about dermatophytes.
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影响因子：
5.5
作者：
Mowlds, Peter;Kavanagh, Kevin
通讯作者：
Kavanagh, Kevin
影响因子：
3.7
作者：
Arendrup MC;Mavridou E;Mortensen KL;Snelders E;Frimodt-Møller N;Khan H;Melchers WJ;Verweij PE
通讯作者：
Verweij PE
影响因子：
9.4
作者：
Fernández-Torres, B;Cabañes, FJ;Guarro, J
通讯作者：
Guarro, J
烟曲霉的分生物色突变体对异源昆虫宿主Galleria mellonella具有高度致病性。
影响因子：
3.7
作者：
Jackson JC;Higgins LA;Lin X
通讯作者：
Lin X
影响因子：
5.2
作者：
Coelho, Luciene M.;Aquino-Ferreira, Roseli;Martinez-Rossi, Nilce M.
通讯作者：
Martinez-Rossi, Nilce M.