QTL mapping of genome regions controlling temephos resistance in larvae of the mosquito Aedes aegypti.
QTL mapping of genome regions controlling temephos resistance in larvae of the mosquito Aedes aegypti.
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DOI：
10.1371/journal.pntd.0003177
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发表时间：
2014-10
影响因子：
3.8
通讯作者：
Black WC 4th
中科院分区：
文献类型：
--
作者：
Reyes-Solis Gdel C;Saavedra-Rodriguez K;Suarez AF;Black WC 4th
埃及伊蚊是登革热和黄热病黄病毒的主要媒介。双硫磷是一种有机磷杀虫剂，在全球范围内用于抑制伊蚊。在许多地方，埃及伊蚊幼虫种群但抗性已经进化。控制双硫磷存活的数量性状位点（QTL）。埃及伊蚊幼虫在来自Solidaridad， m<s:1> xico的双双磷抗性亲本和来自秘鲁伊基托斯的双双磷敏感亲本的F3高级杂交系中被定位。将两组200只F3幼虫暴露于双硫磷鉴别剂量下，每隔2小时至16小时收集死亡幼虫进行DNA分离。存活超过16小时的幼虫被认为具有抗药性。在已知物理位置的23个单拷贝基因和26个微卫星位点上鉴定出单核苷酸多态性（snp）。蚊基因组。在两个互反杂交中，多重间隔定位鉴定出11个与死亡时间相关的QTL。在Solidaridad×Iquitos （SLD×Iq）杂交中，有12个QTL与生存相关，而在IqxSLD互交中，只有6个QTL与生存相关。乙酰胆碱酯酶（AchE）位点1和2的多态性与任何一种抗性表型都没有相关性，这表明靶位不敏感不是该区域的有机磷抗性机制。双双磷抗性受许多影响较小的代谢基因控制，分布在整个白蛉体内。蚊基因组。埃及伊蚊是登革热和黄热病黄病毒的主要媒介。由于缺乏有效的药物或疫苗，如果在不久的将来发生登革热流行，第一道防线将涉及使用杀虫剂来抑制成年伊蚊种群。蚊。不幸的是，这个物种已经对大多数可以安全使用的杀虫剂产生了抗药性。作者广泛研究了伊蚊对各种常用杀虫剂的抗性机制。蚊数量。双硫磷是一种有机磷杀虫剂，在全球范围内用于抑制伊蚊。在许多地方，埃及伊蚊幼虫种群但抗性已经进化。本研究表明，双双磷抗性受多种影响较小的代谢基因控制，分布在整个白纹伊蚊体内。蚊基因组。这一信息对从事野外伊蚊种群抑制工作的野外工作者具有普遍意义。蚊。
The mosquito Aedes aegypti is the principal vector of dengue and yellow fever flaviviruses. Temephos is an organophosphate insecticide used globally to suppress Ae. aegypti larval populations but resistance has evolved in many locations. Quantitative Trait Loci (QTL) controlling temephos survival in Ae. aegypti larvae were mapped in a pair of F3 advanced intercross lines arising from temephos resistant parents from Solidaridad, México and temephos susceptible parents from Iquitos, Peru. Two sets of 200 F3 larvae were exposed to a discriminating dose of temephos and then dead larvae were collected and preserved for DNA isolation every two hours up to 16 hours. Larvae surviving longer than 16 hours were considered resistant. For QTL mapping, single nucleotide polymorphisms (SNPs) were identified at 23 single copy genes and 26 microsatellite loci of known physical positions in the Ae. aegypti genome. In both reciprocal crosses, Multiple Interval Mapping identified eleven QTL associated with time until death. In the Solidaridad×Iquitos (SLD×Iq) cross twelve were associated with survival but in the reciprocal IqxSLD cross, only six QTL were survival associated. Polymorphisms at acetylcholine esterase (AchE) loci 1 and 2 were not associated with either resistance phenotype suggesting that target site insensitivity is not an organophosphate resistance mechanism in this region of México. Temephos resistance is under the control of many metabolic genes of small effect and dispersed throughout the Ae. aegypti genome. The mosquito Aedes aegypti is the principal vector of dengue and yellow fever flaviviruses. Due to a lack of effective drugs or vaccines, if an epidemic of dengue fever occurs in the near future, the first line of defense will involve the use of insecticides to suppress adult populations of Ae. aegypti. Unfortunately, the species has become resistant to most of the insecticides that can be safely applied. The authors have worked extensively on the mechanisms of resistance to the various insecticides commonly used for suppression of Ae. aegypti populations. Temephos is an organophosphate insecticide used globally to suppress Ae. aegypti larval populations but resistance has evolved in many locations. In this study we show that temephos resistance is under the control of many metabolic genes of small effect and dispersed throughout the Ae. aegypti genome. This information will be of general interest to field workers involved in the suppression of field populations of Ae. aegypti.
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影响因子：
3.1
作者：
MUNSTERMANN, LE;CRAIG, GB
通讯作者：
CRAIG, GB
DOI：
10.1590/s0074-02762003000500020
发表时间：
2003-07-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Macoris, Maria de Lourdes G;Andrighetti, Maria Teresa M;Bracco, José Eduardo
通讯作者：
Bracco, José Eduardo
影响因子：
1
作者：
Lozano-Fuentes, Saul;Saavedra-Rodriguez, Karla;Eisen, Lars
通讯作者：
Eisen, Lars
影响因子：
1.8
作者：
Beserra, Eduardo B.;Fernandes, Carlos R.M.;Castro Jr., Francisco P. de
通讯作者：
Castro Jr., Francisco P. de
DOI：
10.1590/s0074-02762004000200015
发表时间：
2004-03-01
期刊：
Memórias do Instituto Oswaldo Cruz
影响因子：
--
作者：
Braga, Ima Aparecida;Lima, José Bento Pereira;Valle, Denise
通讯作者：
Valle, Denise