Modulation of motor behavior by dopamine and the D1-like dopamine receptor AmDOP2 in the honey bee.
Modulation of motor behavior by dopamine and the D1-like dopamine receptor AmDOP2 in the honey bee.
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DOI：
10.1016/j.jinsphys.2009.11.018
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发表时间：
2010-04
影响因子：
2.2
通讯作者：
Smith, Brian H.
中科院分区：
文献类型：
--
作者：
Mustard, Julie A.;Pham, Priscilla M.;Smith, Brian H.
由于存在与不同第二信使途径耦合的多种多巴胺受体亚型，确定多巴胺影响行为的特定分子途径变得复杂。通过观察竞技场中自由移动的成年蜜蜂，研究多巴胺信号传导在调节蜜蜂行为中的作用。多巴胺或多巴胺受体拮抗剂氟哌噻吨被注射到工蜂的血淋巴中。治疗蜂和对照蜂之间的所有行为（行走、停止、倒立、梳理、飞行和扇动）均存在显着差异，并且行为变化取决于药物剂量和注射后的时间。为了检查大脑中特定多巴胺受体的多巴胺信号传导的作用，使用 RNA 干扰来降低 D1 样受体 AmDOP2 的表达水平。将 Amdop2 dsRNA 注射到蘑菇体中会降低 Amdop2 mRNA 的水平，并使蜜蜂执行特定行为的时间发生显着变化，步行时间的减少被梳理毛发或停止时间的增加所抵消。综上所述，这些结果表明多巴胺在调节蜜蜂的运动行为中发挥着重要作用。
Determining the specific molecular pathways through which dopamine affects behavior has been complicated by the presence of multiple dopamine receptor subtypes that couple to different second messenger pathways. The observation of freely moving adult bees in an arena was used to investigate the role of dopamine signaling in regulating the behavior of the honey bee. Dopamine or the dopamine receptor antagonist flupenthixol was injected into the hemolymph of worker honey bees. Significant differences between treated and control bees were seen for all behaviors (walking, stopped, upside down, grooming, flying and fanning), and behavioral shifts were dependent on drug dosage and time after injection. To examine the role of dopamine signaling through a specific dopamine receptor in the brain, RNA interference was used to reduce expression levels of a D1-like receptor, AmDOP2. Injection of Amdop2 dsRNA into the mushroom bodies reduced the levels of Amdop2 mRNA and produced significant changes in the amount of time honey bees spent performing specific behaviors with reductions in time spent walking offset by increases in grooming or time spent stopped. Taken together these results establish that dopamine plays an important role in regulating motor behavior of the honey bee.
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影响因子：
48
作者：
Kulkarni, Meghana M.;Booker, Matthew;Mathey-Prevot, Bernard
通讯作者：
Mathey-Prevot, Bernard
DOI：
10.1073/pnas.0610506104
发表时间：
2007-01-30
影响因子：
11.1
作者：
Barron, Andrew B.;Maleszka, Ryszard;Robinson, Gene E.
通讯作者：
Robinson, Gene E.
DOI：
10.1007/s00359-006-0179-5
发表时间：
2007-02-01
影响因子：
2.1
作者：
Bloch, Guy;Meshi, Avital
通讯作者：
Meshi, Avital
影响因子：
5.3
作者：
Kume, K;Kume, S;Jackson, FR
通讯作者：
Jackson, FR
影响因子：
2.8
作者：
Eban-Rothschild, Ada D.;Bloch, Guy
通讯作者：
Bloch, Guy