Seroprevalence of anti-SARS-CoV-2 antibodies in COVID-19 patients and healthy volunteers up to 6 months post disease onset.
Seroprevalence of anti-SARS-CoV-2 antibodies in COVID-19 patients and healthy volunteers up to 6 months post disease onset.
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DOI：
10.1002/eji.202048970
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发表时间：
2020-12
影响因子：
5.4
通讯作者：
Veldhoen M
中科院分区：
文献类型：
--
作者：
Figueiredo-Campos P;Blankenhaus B;Mota C;Gomes A;Serrano M;Ariotti S;Costa C;Nunes-Cabaço H;Mendes AM;Gaspar P;Pereira-Santos MC;Rodrigues F;Condeço J;Escoval MA;Santos M;Ramirez M;Melo-Cristino J;Simas JP;Vasconcelos E;Afonso Â;Veldhoen M
SARS-CoV-2 已成为一种人类病原体，可引起从发烧到肺炎（COVID-19）等临床症状，但可能仍然轻微或无症状。为了了解病毒的持续传播，检测感染者和曾经感染者，并纵向跟踪免疫反应，需要可靠且稳健的 SARS-CoV-2 检测和免疫监测检测方法。我们在 COVID-19 发病后 6 个月内对识别 SARS-CoV-2 受体结合域 (RBD) 或刺突 (S) 蛋白的 IgM、IgG 和 IgA 抗体进行了定量。我们报告了监测 300 多名 COVID-19 医院患者和医护人员、2500 名大学工作人员以及 198 名 COVID-19 后志愿者的体液免疫反应的详细设置。抗 SARS-CoV-2 抗体反应遵循经典模式，在出现症状后的前三周内迅速增加。尽管滴度随后降低，但检测抗 SARS-CoV-2 IgG 抗体的能力仍然强劲，在大部分先前病毒阳性筛查受试者中具有长达 6 个月的中和活性。我们的工作提供了所用检测方法的详细信息，有助于对 SARS-CoV-2 的保护性免疫进行进一步的纵向分析。重要的是，它强调了大多数确诊的 SARS-CoV-2 患者中循环中和抗体的持续水平。描述了多功能 COVID19 血清学 ELISA 系统的设置，允许通过两步法进行是/否筛选，从而减少假阳性。准确的抗体滴度筛查表明了抗体产生的经典模式，在感染后 3 周达到峰值，并且在 90% 的测试对象中至少 6 个月内保持可检测到。抗体反应与年龄无关，但与疾病的严重程度相关，并且在男性中最初表现出较高的反应。
SARS‐CoV‐2 has emerged as a human pathogen, causing clinical signs, from fever to pneumonia—COVID‐19—but may remain mild or asymptomatic. To understand the continuing spread of the virus, to detect those who are and were infected, and to follow the immune response longitudinally, reliable and robust assays for SARS‐CoV‐2 detection and immunological monitoring are needed. We quantified IgM, IgG, and IgA antibodies recognizing the SARS‐CoV‐2 receptor‐binding domain (RBD) or the Spike (S) protein over a period of 6 months following COVID‐19 onset. We report the detailed setup to monitor the humoral immune response from over 300 COVID‐19 hospital patients and healthcare workers, 2500 University staff, and 198 post‐COVID‐19 volunteers. Anti‐SARS‐CoV‐2 antibody responses follow a classic pattern with a rapid increase within the first three weeks after symptoms. Although titres reduce subsequently, the ability to detect anti‐SARS‐CoV‐2 IgG antibodies remained robust with confirmed neutralization activity for up to 6 months in a large proportion of previously virus‐positive screened subjects. Our work provides detailed information for the assays used, facilitating further and longitudinal analysis of protective immunity to SARS‐CoV‐2. Importantly, it highlights a continued level of circulating neutralising antibodies in most people with confirmed SARS‐CoV‐2. The setup of a versatile COVID19 serology ELISA system is described, allowing Yes/No screening with a two‐step method that reduces false‐positives. Accurate antibody titer screening indicates a classic pattern of antibody production, peaking at 3 weeks post‐infection and remaining detectable for at least 6 months in 90% of the subjects tested. The antibody response does not correlate with age, but with severity of disease, and show an initial higher response in men.
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DOI：
10.1056/nejmoa2022926
发表时间：
2020-11-19
期刊：
The New England journal of medicine
影响因子：
--
作者：
RECOVERY Collaborative Group;Horby P;Mafham M;Linsell L;Bell JL;Staplin N;Emberson JR;Wiselka M;Ustianowski A;Elmahi E;Prudon B;Whitehouse T;Felton T;Williams J;Faccenda J;Underwood J;Baillie JK;Chappell LC;Faust SN;Jaki T;Jeffery K;Lim WS;Montgomery A;Rowan K;Tarning J;Watson JA;White NJ;Juszczak E;Haynes R;Landray MJ
通讯作者：
Landray MJ
影响因子：
82.9
作者：
Long, Quan-Xin;Liu, Bai-Zhong;Huang, Ai-Long
通讯作者：
Huang, Ai-Long
影响因子：
168.9
作者：
Pollan, Marina;Perez-Gomez, Beatriz;Yotti, Raquel
通讯作者：
Yotti, Raquel
影响因子：
--
作者：
Stadlbauer, Daniel;Amanat, Fatima;Krammer, Florian
通讯作者：
Krammer, Florian
影响因子：
16.6
作者：
GeurtsvanKessel, Corine H.;Okba, Nisreen M. A.;Koopmans, Marion
通讯作者：
Koopmans, Marion