A Putative D-Arabinono-1,4-lactone Oxidase, MoAlo1, Is Required for Fungal Growth, Conidiogenesis, and Pathogenicity in Magnaporthe oryzae.
A Putative D-Arabinono-1,4-lactone Oxidase, MoAlo1, Is Required for Fungal Growth, Conidiogenesis, and Pathogenicity in Magnaporthe oryzae.
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稻瘟病菌的真菌生长、分生孢子发生和致病性需要一种推定的 D-Arabinono-1,4-内酯氧化酶 MoAlo1。
DOI：
10.3390/jof8010072
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发表时间：
2022-01-11
期刊：
影响因子：
--
通讯作者：
Liu XH
中科院分区：
文献类型：
--
作者：
Wu MH;Huang LY;Sun LX;Qian H;Wei YY;Liang S;Zhu XM;Li L;Lu JP;Lin FC;Liu XH
稻瘟病菌是水稻稻瘟病暴发的病原。L-抗坏血酸（ASC）是自然界中一种著名的抗氧化剂。然而，虽然ASC在真菌中是罕见或不存在的，但五碳类似物D-赤式抗坏血酸（EASC）似乎是ASC的替代品。虽然ASC的抗氧化功能已被广泛描述，但EASC的具体性质和生理功能仍然知之甚少。在这项研究中，我们确定了一个D-阿拉伯糖-1，4-内酯氧化酶（ALO）结构域的蛋白，MoAlo 1，并发现MoAlo 1定位于线粒体。MoALO 1（Δ Moalo 1）的破坏表现出营养生长以及分生孢子发生的缺陷。发现Δ Moalo 1突变体对外源H2 O2更敏感。此外，Δ Moalo 1缺失突变体的分生孢子在水稻中的致病性显著降低，菌丝顶端形成的附着胞样结构（ALS）穿透能力也有缺陷。当在孢子悬浮液中加入外源EASC后，Δ Moalo 1突变体的致病性得到恢复。MoAlo 1对M.米。
Magnaporthe oryzae is the causal agent of rice blast outbreaks. L-ascorbic acid (ASC) is a famous antioxidant found in nature. However, while ASC is rare or absent in fungi, a five-carbon analog, D-erythroascorbic acid (EASC), seems to appear to be a substitute for ASC. Although the antioxidant function of ASC has been widely described, the specific properties and physiological functions of EASC remain poorly understood. In this study, we identified a D-arabinono-1,4-lactone oxidase (ALO) domain-containing protein, MoAlo1, and found that MoAlo1 was localized to mitochondria. Disruption of MoALO1 (ΔMoalo1) exhibited defects in vegetative growth as well as conidiogenesis. The ΔMoalo1 mutant was found to be more sensitive to exogenous H2O2. Additionally, the pathogenicity of conidia in the ΔMoalo1 null mutant was reduced deeply in rice, and defective penetration of appressorium-like structures (ALS) formed by the hyphal tips was also observed in the ΔMoalo1 null mutant. When exogenous EASC was added to the conidial suspension, the defective pathogenicity of the ΔMoalo1 mutant was restored. Collectively, MoAlo1 is essential for growth, conidiogenesis, and pathogenicity in M. oryzae.
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影响因子：
56.9
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