A functional proteomic method for biomarker discovery.
A functional proteomic method for biomarker discovery.
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DOI：
10.1371/journal.pone.0022471
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Kelly KA
中科院分区：
文献类型：
--
作者：
Reynolds F;Panneer N;Tutino CM;Wu M;Skrabal WR;Moskaluk C;Kelly KA
The sequencing of the human genome holds out the hope for personalized medicine, but it is clear that analysis of DNA or RNA content alone is not sufficient to understand most disease processes. Proteomic strategies that allow unbiased identification of proteins and their post-transcriptional and -translation modifications are an essential complement to genomic strategies. However, the enormity of the proteome and limitations in proteomic methods make it difficult to determine the targets that are particularly relevant to human disease. Methods are therefore needed that allow rational identification of targets based on function and relevance to disease. Screening methodologies such as phage display, SELEX, and small-molecule combinatorial chemistry have been widely used to discover specific ligands for cells or tissues of interest, such as tumors. Those ligands can be used in turn as affinity probes to identify their cognate molecular targets when they are not known in advance. Here we report an easy, robust and generally applicable approach in which phage particles bearing cell- or tissue-specific peptides serve directly as the affinity probes for their molecular targets. For proof of principle, the method successfully identified molecular binding partners, three of them novel, for 15 peptides specific for pancreatic cancer.
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影响因子：
3.2
作者：
Bausch, Dirk;Mino-Kenudson, Mari;Fernandez-del Castillo, Carlos;Warshaw, Andrew L.;Kelly, Kimberly A.;Thayer, Sarah P.
通讯作者：
Thayer, Sarah P.
影响因子：
20.3
作者：
Das, Riku;Burke, Tim;Plow, Edward F.
通讯作者：
Plow, Edward F.
影响因子：
2.9
作者：
Herren, Thomas;Burke, Timothy A.;Plow, Edward F.
通讯作者：
Plow, Edward F.
影响因子：
2.2
作者：
Davis, RG;Vishwanatha, JK
通讯作者：
Vishwanatha, JK
DOI：
10.1007/s11805-009-0313-6
发表时间：
2009-10-01
期刊：
Clinical Oncology and Cancer Research
影响因子：
--
作者：
Han, Haibo;Du, Yantao;Wei, Zhao
通讯作者：
Wei, Zhao