A method to improve the efficacy of topical eflornithine hydrochloride cream.
A method to improve the efficacy of topical eflornithine hydrochloride cream.
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DOI：
10.3109/10717544.2014.951746
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发表时间：
2016-06
期刊：
影响因子：
6
通讯作者：
Cui Z
中科院分区：
文献类型：
--
作者：
Kumar A;Naguib YW;Shi YC;Cui Z
面部多毛症是女性的一个美容问题，会导致严重的焦虑和缺乏自尊。依氟鸟氨酸乳膏用于治疗面部多毛症。然而，即使在长期和高频率的应用中，有限的成功率和总体患者满意度也留下了改进的空间。本研究的目的是在小鼠模型上测试微针治疗对依氟鸟氨酸乳膏体外皮肤渗透的影响和体内疗效。采用Franz扩散池对依氟鸟氨酸进行体外渗透研究。用微针将依氟鸟氨酸乳膏涂于小鼠下背皮肤，观察其毛发再生情况，建立小鼠模型进行体内疗效研究。对治疗区皮肤和毛囊进行组织学检查。当将依氟鸟氨酸乳膏涂抹在用微针预处理过的小鼠皮肤区域时，依氟鸟氨酸的毛发生长抑制活性显著增强，很可能是因为微针产生的微孔允许依氟鸟氨酸渗透到皮肤中，这在体外渗透研究中得到了证实。免疫组织化学数据显示，将依氟鸟氨酸乳膏涂抹在微针预处理的皮肤区域，皮肤和毛囊的细胞增殖也明显受到抑制。将微针治疗整合到局部依氟鸟氨酸治疗中代表了一种潜在可行的方法来增加依氟鸟氨酸抑制头发生长的能力。
Facial hirsutism is a cosmetic concern for women and can lead to significant anxiety and lack of self-esteem. Eflornithine cream is indicated for the treatment of facial hirsutism. However, limited success rate and overall patient’s satisfaction, even with a long-term and high frequency application, leave room for improvement. The objective of this study is to test the effect of microneedle treatment on the in vitro skin permeation and the in vivo efficacy of eflornithine cream in a mouse model. In vitro permeation study of eflornithine was performed using Franz diffusion cell. In vivo efficacy study was performed in a mouse model by monitoring the re-growth of hair in the lower dorsal skin of mice after the eflornithine cream was applied onto an area pretreated with microneedles. The skin and the hair follicles in the treated area were also examined histologically. The hair growth inhibitory activity of eflornithine was significantly enhanced when the eflornithine cream was applied onto a mouse skin area pretreated with microneedles, most likely because the micropores created by microneedles allowed the permeation of eflornithine into the skin, as confirmed in an in vitro permeation study. Immunohistochemistry data revealed that cell proliferation in the skin and hair follicles was also significantly inhibited when the eflornithine cream was applied onto a skin area pretreated with microneedles. The integration of microneedle treatment into topical eflornithine therapy represents a potentially viable approach to increase eflornithine’s ability to inhibit hair growth.
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影响因子：
8
作者：
Kumar A;Li X;Sandoval MA;Rodriguez BL;Sloat BR;Cui Z
通讯作者：
Cui Z
影响因子：
14.5
作者：
Naguib, Youssef W.;Kumar, Amit;Cuin, Zhengrong
通讯作者：
Cuin, Zhengrong
影响因子：
5.7
作者：
Kaushik, S;Hord, AH;Prausnitz, MR
通讯作者：
Prausnitz, MR
影响因子：
2
作者：
Burri, C;Brun, R
通讯作者：
Brun, R
影响因子：
4.6
作者：
Badran, M. M.;Kuntsche, J.;Fahr, A.
通讯作者：
Fahr, A.