Growth temperature-dependent variation in the bacteriophage-inactivating capacity and antigenicity of Yersinia enterocolitica lipopolysaccharide.
Growth temperature-dependent variation in the bacteriophage-inactivating capacity and antigenicity of Yersinia enterocolitica lipopolysaccharide.
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小肠结肠炎耶尔森氏菌脂多糖的噬菌体灭活能力和抗原性随生长温度的变化。
DOI：
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
M. Tsubokura
中科院分区：
文献类型：
--
作者：
Y. Kawaoka;K. Otsuki;M. Tsubokura
生长温度影响小肠结肠炎耶尔森菌Ye 3827脂多糖（LPS）的结构。虽然Y.小肠结肠炎Ye 3827在低温（25摄氏度）生长时合成光滑的LPS，在生理温度（37摄氏度）生长时发生部分光滑-粗糙转变。通过噬菌体灭活试验和化学及免疫学分析检测结构改变。由在25 ℃下生长的细菌制备的LPS灭活了许多识别LPS的O-抗原多糖部分的噬菌体，而对于相同程度的灭活，需要37 ℃下生长的细菌的LPS的量的3000倍以上。负责25 ℃-LPS和抗25 ℃-细菌之间主要反应的抗原决定簇位于LPS的O-抗原多糖部分，而负责37 ℃-LPS和抗37 ℃-细菌之间主要反应的抗原决定簇位于LPS的R-核心或内部。
Growth temperature affected the structure of Yersinia enterocolitica Ye 3827 lipopolysaccharide (LPS). Although Y. enterocolitica Ye 3827 synthesized smooth LPS when grown at a low temperature (25 degrees C), partial smooth-rough transition occurred when the bacteria were grown at the physiological temperature (37 degrees C). The structural alteration was detected by bacteriophage-inactivation assay and chemical and immunological analyses. LPS prepared from bacteria grown at 25 degrees C inactivated a number of bacteriophages that recognize the O-antigenic polysaccharide portion of LPS, whereas more than 3000 times the amount of LPS from bacteria grown at 37 degrees C was required for the same degree of inactivation. The antigenic determinant(s) responsible for the major reaction between 25 degrees C-LPS and anti-25 degree C-bacteria was located on the O-antigenic polysaccharide portion of LPS, but those responsible for the major reaction between 37 degrees C-LPS and anti-37 degrees C-bacteria were located on the R-core or inner portion of LPS.
在模拟哺乳动物细胞内环境的条件下培养的耶尔森氏菌的细胞质和膜蛋白。
DOI：
10.1073/pnas.78.2.1224
发表时间：
1981
影响因子：
11.1
作者：
Straley,SC;Brubaker,RR
通讯作者：
Brubaker,RR