Effects of HuoxueJiangtang decoction alone or in combination with metformin on renal function and renal cortical mRNA expression in diabetic nephropathy rats.
Effects of HuoxueJiangtang decoction alone or in combination with metformin on renal function and renal cortical mRNA expression in diabetic nephropathy rats.
复制标题
活血降糖汤单用或联合二甲双胍对糖尿病肾病大鼠肾功能及肾皮质mRNA表达的影响
DOI：
10.1080/13880209.2020.1844242
复制
发表时间：
2020-12
影响因子：
3.8
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Liu X;Liu D;Shuai Y;Li H;Zhao H;Qu X;Chu S;Zhang X
活血降糖汤是治疗糖尿病的传统中药。目的观察中药ZY对糖尿病肾病大鼠肾损伤的保护作用。材料与方法选用4周龄SPF级Wistar雄性大鼠50只，连续高脂喂养4周，尾静脉注射30 mg/kg链脲佐菌素1周，建立糖尿病肾病模型组（DN组）。实验分为正常对照组、DN组、DN + ZY组、DN +二甲双胍组、DN +二甲双胍+ ZY组和DN +二甲双胍+卡托普利组。其中ZY 6.25 g/kg、二甲双胍250 mg/kg、卡托普利17.5 mg/kg灌胃给药，每日1次，连续16周。测量各组的血糖、饮食行为、生化指标和基因表达变化。结果二甲双胍+ ZY治疗能显著降低DN组的血糖、饮水量、尿总蛋白、尿白蛋白、尿量、血清甘油三酯、血清胆固醇水平。肾组织病理学变化显示，DN +二甲双胍+ ZY组对肾组织损伤具有保护作用。ZY和二甲双胍+ ZY治疗组DN组基因表达有修复作用。讨论与结论ZY联合二甲双胍治疗DN肾损害有明显疗效。本研究为ZY联合二甲双胍治疗糖尿病肾病提供了潜在的机制。
Abstract Context HuoxueJiangtang decoction (ZY) is a traditional Chinese medicine for the treatment of diabetes. Objective The protective effect of ZY on renal injury in diabetic nephropathy rats was investigated in this study. Materials and methods Fifty 4-week-old SPF Wistar male rats were selected to construct diabetic nephropathy model rats (DN) group by continuous high-fat feeding for 4 weeks, followed by a tail vein injection of 30 mg/kg streptozotocin for 1 week. The experimental rats were divided into six groups of 10 rats: normal (control), DN, DN + ZY, DN + metformin, DN + metformin + ZY, and DN + metformin + captopril (positive control) groups. Among the groups, 6.25 g/kg ZY, 250 mg/kg metformin, and 17.5 mg/kg captopril were given to the rats by gavage once a day for 16 weeks. Blood glucose, dietary behaviour, biochemical indicators, and gene expression changes were measured in each group. Results Metformin + ZY treatment significantly lowered blood glucose, water intake, urine total protein, urine albumin, urine volume, serum triglyceride, and serum cholesterol levels in the DN group. The pathological changes of kidney tissue showed that the DN + metformin + ZY group had a protective effect on kidney tissue damage. And ZY and metformin + ZY treatments repaired the expression of genes in the DN group. Discussion and conclusion The ZY and metformin combined treatment showed a clear therapeutic effect on kidney damage in DN. This study provides a potential mechanism for the treatment of diabetic nephropathy with ZY combined with metformin.
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DOI：
10.1056/nejmoa1114248
发表时间：
2012-07-05
期刊：
The New England journal of medicine
影响因子：
--
作者：
Inker LA;Schmid CH;Tighiouart H;Eckfeldt JH;Feldman HI;Greene T;Kusek JW;Manzi J;Van Lente F;Zhang YL;Coresh J;Levey AS;CKD-EPI Investigators
通讯作者：
CKD-EPI Investigators
影响因子：
3
作者：
Alpers CE;Hudkins KL
通讯作者：
Hudkins KL
影响因子：
6.1
作者：
Schwartz, MM;Lewis, EJ;Batlle, D
通讯作者：
Batlle, D
影响因子：
4.8
作者：
Tominaga, Tatsuya;Dutta, Rajesh K.;Kanwar, Yashpal S.
通讯作者：
Kanwar, Yashpal S.
DOI：
10.1016/j.pnpbp.2013.10.006
发表时间：
2014-01-03
影响因子：
5.6
作者：
Boyda, Heidi N.;Procyshyn, Ric M.;Barr, Alasdair M.
通讯作者：
Barr, Alasdair M.