Costs of reproduction are present but latent in eusocial bumblebee queens.
Costs of reproduction are present but latent in eusocial bumblebee queens.
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繁殖的成本存在，但在欧洲大黄蜂皇后区潜在。
DOI：
10.1186/s12915-023-01648-5
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发表时间：
2023-07-10
期刊：
影响因子：
5.4
通讯作者：
Bourke, Andrew F. G.
中科院分区：
文献类型：
--
作者：
Collins, David H.;Prince, David C.;Donelan, Jenny L.;Chapman, Tracey;Bourke, Andrew F. G.
关键词：
衰老的标准进化论认为，衰老是在生殖和寿命之间权衡的结果。社会性昆虫女王表现出正的繁殖力-长寿关系，因此被认为是反例，因为它们不表达繁殖成本，而是通过重塑调节衰老和生殖的保守的遗传和内分泌网络。如果是这样的话，从具有负繁殖力和长寿关系的孤独祖先开始的社会进化肯定涉及到一个阶段，在这个阶段，繁殖成本被抑制，繁殖力和长寿变得正相关。使用大黄蜂(Bombus Terrestris)，我们通过实验测试了处于中等社会复杂性水平的一年生真社会昆虫中的女王是否经历了繁殖成本，并使用mRNA-seq，测试了它们表现出相关遗传和内分泌网络重构的程度。具体地说，我们测试了繁殖成本是否存在但潜在的，或者是否已经发生了相关基因和内分泌网络的重构，允许蚁后免费繁殖。我们在实验中通过移除蜂王的卵来增加蜂王的繁殖成本，这会导致蜂王增加它们的产卵率。与产蛋率没有提高的对照蜂王相比，处理蜂王的寿命显著降低。治疗后寿命的下降并不是由于工作人员对女王的攻击性增加或女王整体活动的增加造成的。此外，处理蜂王和对照蜂王在基于mRNA-SEQ的年龄相关基因表达上存在差异，它们的总体表达谱和年龄相关基因的表达都不同。值得注意的是，这些差异似乎主要发生在相对年龄上，而不是年龄上。这项研究代表了第一次同时进行表型和转录的实验测试，以确定真社会性昆虫女王的繁殖寿命成本。这些结果支持中等社会复杂性的一年生真社会性昆虫的繁殖成本的发生，并表明繁殖成本在这类物种的蜂王中是存在的但潜在的，即这些蜂王表现出条件依赖的正繁殖力-寿命关联。它们还提出了这样一种可能性，即支撑衰老的遗传和内分泌网络的部分重构可能发生在中等社会性物种中，因此，在未经处理的条件下，与年龄相关的基因表达更多地依赖于时间而不是相对年龄。网上版载有补充材料，可在10.1186/s12915-023-01648-5查阅。
The standard evolutionary theory of ageing proposes that ageing occurs because of a trade-off between reproduction and longevity. Eusocial insect queens exhibit positive fecundity-longevity associations and so have been suggested to be counter-examples through not expressing costs of reproduction and through remodelling conserved genetic and endocrine networks regulating ageing and reproduction. If so, eusocial evolution from solitary ancestors with negative fecundity-longevity associations must have involved a stage at which costs of reproduction were suppressed and fecundity and longevity became positively associated. Using the bumblebee (Bombus terrestris), we experimentally tested whether queens in annual eusocial insects at an intermediate level of eusocial complexity experience costs of reproduction, and, using mRNA-seq, the extent to which they exhibit a remodelling of relevant genetic and endocrine networks. Specifically, we tested whether costs of reproduction are present but latent, or whether a remodelling of relevant genetic and endocrine networks has already occurred allowing queens to reproduce without costs. We experimentally increased queens’ costs of reproduction by removing their eggs, which caused queens to increase their egg-laying rate. Treatment queens had significantly reduced longevity relative to control queens whose egg-laying rate was not increased. Reduced longevity in treatment queens was not caused by increased worker-to-queen aggression or by increased overall activity in queens. In addition, treatment and control queens differed in age-related gene expression based on mRNA-seq in both their overall expression profiles and the expression of ageing-related genes. Remarkably, these differences appeared to occur principally with respect to relative age, not chronological age. This study represents the first simultaneously phenotypic and transcriptomic experimental test for a longevity cost of reproduction in eusocial insect queens. The results support the occurrence of costs of reproduction in annual eusocial insects of intermediate social complexity and suggest that reproductive costs are present but latent in queens of such species, i.e. that these queens exhibit condition-dependent positive fecundity-longevity associations. They also raise the possibility that a partial remodelling of genetic and endocrine networks underpinning ageing may have occurred in intermediately eusocial species such that, in unmanipulated conditions, age-related gene expression depends more on chronological than relative age. The online version contains supplementary material available at 10.1186/s12915-023-01648-5.
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影响因子：
4.9
作者：
Collins DH;Wirén A;Labédan M;Smith M;Prince DC;Mohorianu I;Dalmay T;Bourke AFG
通讯作者：
Bourke AFG
影响因子：
2
作者：
HAMILTON, WD
通讯作者：
HAMILTON, WD
影响因子：
5.2
作者：
Cohen, Alan A.;Coste, Christophe F. D.;Pavard, Samuel
通讯作者：
Pavard, Samuel
DOI：
10.1098/rspb.2017.0380
发表时间：
2017-07-12
期刊：
Proceedings. Biological sciences
影响因子：
--
作者：
Blacher P;Huggins TJ;Bourke AFG
通讯作者：
Bourke AFG
影响因子：
4.9
作者：
Costa, Claudineia P.;Duennes, Michelle A.;Woodard, S. Hollis
通讯作者：
Woodard, S. Hollis