Gene expression during larval caste determination and differentiation in intermediately eusocial bumblebees, and a comparative analysis with advanced eusocial honeybees.
Gene expression during larval caste determination and differentiation in intermediately eusocial bumblebees, and a comparative analysis with advanced eusocial honeybees.
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DOI：
10.1111/mec.15752
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发表时间：
2021-03
影响因子：
4.9
通讯作者：
Bourke AFG
中科院分区：
文献类型：
--
作者：
Collins DH;Wirén A;Labédan M;Smith M;Prince DC;Mohorianu I;Dalmay T;Bourke AFG
真社会性昆虫的蚁后-工蚁种姓制度代表了发育多型现象（环境诱导的表型多态性）的一个典型例子，并且是高级真社会性进化的内在特征。然而，真社会性膜翅目幼虫种姓确定和随后分化的比较分子基础仍然知之甚少。为了解决蜜蜂中的这个问题，我们分析了中等社会性熊蜂 Bombus terrestris 雌性幼虫中与种姓相关的基因表达。在B. terrestris中，雌性幼虫经历了一个女王依赖期，在此期间它们成年后的种姓命运是决定的，随后是一个营养敏感期，也可能影响种姓命运，但证据较弱。我们使用 mRNA-seq 和 qRT-PCR 验证来分离幼虫在每个时期之前和之后的发育阶段的每个种姓途径之间差异表达的基因。我们发现，在全能幼虫中，种姓途径之间的基因表达差异很小，然后在蜂王依赖期后达到峰值。尽管新基因在工蚁途径中的晚龄幼虫中显着富集，但在种姓之间差异表达的新基因（即分类学限制的）相对较少。我们将 B. terrestris 中的种姓相关基因组与高级社会性蜜蜂 Apis mellifera 中报告的基因组进行了比较，发现两个物种之间的基因列表存在显着但相对较低的重叠。这些结果表明，自最后一个共同的社会性祖先以来，熊蜂和高级社会性蜜蜂之间存在少量的共享工具包基因，并且与种姓相关的基因存在显着差异。
The queen‐worker caste system of eusocial insects represents a prime example of developmental polyphenism (environmentally‐induced phenotypic polymorphism) and is intrinsic to the evolution of advanced eusociality. However, the comparative molecular basis of larval caste determination and subsequent differentiation in the eusocial Hymenoptera remains poorly known. To address this issue within bees, we profiled caste‐associated gene expression in female larvae of the intermediately eusocial bumblebee Bombus terrestris. In B. terrestris, female larvae experience a queen‐dependent period during which their caste fate as adults is determined followed by a nutrition‐sensitive period also potentially affecting caste fate but for which the evidence is weaker. We used mRNA‐seq and qRT‐PCR validation to isolate genes differentially expressed between each caste pathway in larvae at developmental stages before and after each of these periods. We show that differences in gene expression between caste pathways are small in totipotent larvae, then peak after the queen‐dependent period. Relatively few novel (i.e., taxonomically‐restricted) genes were differentially expressed between castes, though novel genes were significantly enriched in late‐instar larvae in the worker pathway. We compared sets of caste‐associated genes in B. terrestris with those reported from the advanced eusocial honeybee, Apis mellifera, and found significant but relatively low levels of overlap of gene lists between the two species. These results suggest both the existence of low numbers of shared toolkit genes and substantial divergence in caste‐associated genes between Bombus and the advanced eusocial Apis since their last common eusocial ancestor.
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影响因子：
4.6
作者：
Collins DH;Mohorianu I;Beckers M;Moulton V;Dalmay T;Bourke AF
通讯作者：
Bourke AF
高度社会性蜜蜂意大利蜜蜂种姓分化的分子决定因素。
影响因子：
--
作者：
Barchuk AR;Cristino AS;Kucharski R;Costa LF;Simões ZL;Maleszka R
通讯作者：
Maleszka R
影响因子：
4.4
作者：
Colgan TJ;Carolan JC;Bridgett SJ;Sumner S;Blaxter ML;Brown MJ
通讯作者：
Brown MJ
影响因子：
4.4
作者：
Elsik, Christine G.;Worley, Kim C.;Gibbs, Richard A.
通讯作者：
Gibbs, Richard A.
DOI：
10.1073/pnas.96.10.5575
发表时间：
1999-05-11
影响因子：
11.1
作者：
Evans, JD;Wheeler, DE
通讯作者：
Wheeler, DE