Neuropeptidomics of the supraoptic rat nucleus.
Neuropeptidomics of the supraoptic rat nucleus.
复制标题
DOI：
10.1021/pr800394e
复制
发表时间：
2008-11
影响因子：
4.4
通讯作者：
Sweedler, Jonathan V.
中科院分区：
文献类型：
--
作者：
Bora, Adriana;Annangudi, Suresh P.;Millet, Larry J.;Rubakhin, Stanislav S.;Forbes, Andrew J.;Kelleher, Neil L.;Gillette, Martha U.;Sweedler, Jonathan V.
关键词：
哺乳动物视上核（SON）是大脑中的一个神经内分泌中心，调节多种生理功能。在视上核内，投射到神经垂体（垂体后叶）的肽能大细胞神经元参与控制渗透平衡、泌乳和分娩，部分是通过向血液中分泌诸如催产素和抗利尿激素等信号肽来实现的。要更好地理解视上核的活动和功能，需要对视上核所使用的肽进行鉴定和表征。在此，针对从整个核到单个大细胞神经元的分离视上核样本的神经肽组学研究，对小体积样本制备方法进行了优化。与以往大多数哺乳动物肽组研究不同，组织不会立即被加热或用微波处理。视上核样本通过组织穿刺从离体脑片制备中获取，样本经过肽提取的连续步骤进行处理。通过液相色谱 - 质谱和串联质谱对样本进行分析，鉴定出85种肽，包括来自已知前激素的20种独特肽。随着样本量进一步减少，肽覆盖的深度降低；然而，即使从单独分离的大细胞神经内分泌细胞中，也能检测到抗利尿激素和其他几种肽。
哺乳动物肽组研究扩展到更小的、定义明确的脑结构，从单个脑核到单个神经元。具体而言，我们对视上核以及核内单个大细胞神经元的肽组进行了表征。通过使用冠状脑片制备和优化的样本制备策略以减少死后降解，已对85种肽进行了表征，包括20种先前未报道的肽。
The mammalian supraoptic nucleus (SON) is a neuroendocrine center in the brain regulating a variety of physiological functions. Within the SON, peptidergic magnocellular neurons that project to the neurohypophysis (posterior pituitary) are involved in controlling osmotic balance, lactation, and parturition, partly through secretion of signaling peptides such as oxytocin and vasopressin into the blood. An improved understanding of SON activity and function requires identification and characterization of the peptides used by the SON. Here, small-volume sample preparation approaches are optimized for neuropeptidomic studies of isolated SON samples ranging from entire nuclei down to single magnocellular neurons. Unlike most previous mammalian peptidome studies, tissues are not immediately heated or microwaved. SON samples are obtained from ex vivo brain slice preparations via tissue punch and the samples processed through sequential steps of peptide extraction. Analyses of the samples via liquid chromatography mass spectrometry and tandem mass spectrometry result in the identification of 85 peptides, including 20 unique peptides from known prohormones. As the sample size is further reduced, the depth of peptide coverage decreases; however, even from individually isolated magnocellular neuroendocrine cells, vasopressin and several other peptides are detected. Mammalian peptidome studies are extended to smaller, well-defined brain structures ranging from an individual brain nucleus to individual neurons. Specifically, we characterize the peptidome of the supraoptic nucleus and individual magnocellular neurons within the nucleus. With the use of a coronal brain slice preparation and optimized sample preparation strategies to reduce postmortem degradation, 85 peptides have been characterized, including 20 peptides previously not reported.
登录
查看更多内容
影响因子：
2.3
作者：
Che, FY;Biswas, R;Fricker, LD
通讯作者：
Fricker, LD
影响因子：
2.5
作者：
Fiala, JC;Kirov, SA;Harris, KM
通讯作者：
Harris, KM
影响因子：
2.9
作者：
Ang, CW;Dotman, CH;VanLeeuwen, FW
通讯作者：
VanLeeuwen, FW
影响因子：
2.9
作者：
BAUER, CA;THOMPSON, RC;BLOUT, ER
通讯作者：
BLOUT, ER
影响因子：
5.5
作者：
BRIMBLE, MJ;DYBALL, REJ;FORSLING, ML
通讯作者：
FORSLING, ML