Quantitative Chemical Imaging of Bone Tissue for Intraoperative and Diagnostic Applications.
Quantitative Chemical Imaging of Bone Tissue for Intraoperative and Diagnostic Applications.
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DOI：
10.1021/acs.analchem.1c04354
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发表时间：
2022-03-08
影响因子：
7.4
通讯作者：
Fu D
中科院分区：
文献类型：
--
作者：
Shin KS;Men S;Wong A;Cobb-Bruno C;Chen EY;Fu D
骨难以使用传统的组织病理学方法进行成像，导致术中病理学评估的挑战，这对于指导手术治疗至关重要，特别是在骨科肿瘤学中。在这项研究中，我们证明了一个多模态的定量成像方法，结合受激拉曼散射（SRS）显微镜，双光子荧光（TPF）显微镜，和二次谐波发生（SHG）显微镜可以提供有用的诊断信息完整的骨组织碎片从手术切除或活检标本。我们对17例患者的骨样本进行了成像，并对骨的无机和有机成分进行了定量化学和形态学分析。我们的主要研究结果表明，碳酸盐含量结合骨有机基质的形态测定分析可以分离出几个主要类别的骨癌相关的诊断类别，平均准确率为92%。这项原理验证研究表明，骨组织的定量多模态成像和基于机器学习的分析可以为指导骨科肿瘤学的临床决策提供关键的诊断信息。此外，形态学和化学成像与机器学习相结合的一般方法可以很容易地扩展到其他组织类型，用于术中和其他临床环境中的组织诊断。
Bone is difficult to image using traditional histopathological methods, leading to challenges in intraoperative pathological evaluation that is critical in guiding surgical treatment, particularly in orthopedic oncology. In this study, we demonstrate that a multimodal quantitative imaging approach that combines stimulated Raman scattering (SRS) microscopy, two-photon fluorescence (TPF) microscopy, and second harmonic generation (SHG) microscopy can provide useful diagnostic information regarding intact bone tissue fragments from surgical excision or biopsy specimens. We imaged bone samples from 17 patient cases and performed quantitative chemical and morphological analyses of both mineral and organic components of bone. Our main findings show that carbonate content combined with morphometric analysis of bone organic matrix can separate several major classes of bone cancer associated diagnostic categories with an average accuracy of 92%. This proof-of-principle study demonstrate that quantitative multimodal imaging and machine learning-based analysis of bony tissue can provide crucial diagnostic information for guiding clinical decisions in orthopedic oncology. Moreover, the general methodology of morphological and chemical imaging combined with machine learning can be readily extended to other tissue types for tissue diagnosis in intraoperative and other clinical settings.
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影响因子：
4.1
作者：
Bi, Xiaohong;Sterling, Julie A.;Merkel, Alyssa R.;Perrien, Daniel S.;Nyman, Jeffry S.;Mahadevan-Jansen, Anita
通讯作者：
Mahadevan-Jansen, Anita
影响因子：
20.4
作者：
Mandrekar, Jayawant N.
通讯作者：
Mandrekar, Jayawant N.
DOI：
10.1002/cyto.990080610
发表时间：
1987-11-01
期刊：
CYTOMETRY
影响因子：
--
作者：
BAUER, HCF;KREICBERGS, A
通讯作者：
KREICBERGS, A
影响因子：
23.8
作者：
Manifold B;Men S;Hu R;Fu D
通讯作者：
Fu D
DOI：
10.1038/labinvest.2017.116
发表时间：
2018-01
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
Cahill LC;Giacomelli MG;Yoshitake T;Vardeh H;Faulkner-Jones BE;Connolly JL;Sun CK;Fujimoto JG
通讯作者：
Fujimoto JG