Spatiotemporal dynamics of lesion-induced axonal sprouting and its relation to functional architecture of the cerebellum.
Spatiotemporal dynamics of lesion-induced axonal sprouting and its relation to functional architecture of the cerebellum.
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DOI：
10.1038/ncomms12938
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发表时间：
2016-09-21
影响因子：
16.6
通讯作者：
Nishiyama, Hiroshi
中科院分区：
文献类型：
--
作者：
Dhar, Matasha;Brenner, Joshua M.;Sakimura, Kenji;Kano, Masanobu;Nishiyama, Hiroshi
神经退行性病变会导致幸存的轴突长出新的侧枝，但这种形式的轴突重塑在多大程度上改变了大脑功能结构仍不清楚。为了了解成人大脑中侧支萌芽的进行情况，我们使用活体延时显微镜对小鼠小脑攀缘纤维（CF）损伤后的萌芽进行了成像。在这里，我们发现新萌芽的 CF 侧枝在几个月内神经支配多个浦肯野细胞 (PC)，大多数神经支配出现在病变后 3-4 周。小脑功能结构的同步成像显示，幸存的 CF 类似地支配功能相关和不相关的 PC，但靠近侧支起源的 PC 上的突触面积比远处的 PC 上的突触面积更多。这些结果表明，新萌芽的轴突侧支不会优先神经支配功能相关的突触后目标。尽管如此，如果功能相关的神经元聚集在病变区域，侧支神经支配的空间梯度可能有助于松散地维持功能性突触回路。 神经退行性病变诱导幸存的轴突发芽，但这些侧枝与现有功能网络相关的重新神经支配模式仍不清楚。在这里，作者对小鼠进行了长期体内成像，对病变后小脑攀缘纤维的芽进行了观察，并描述了与功能活跃区相关的连接模式。
Neurodegenerative lesions induce sprouting of new collaterals from surviving axons, but the extent to which this form of axonal remodelling alters brain functional structure remains unclear. To understand how collateral sprouting proceeds in the adult brain, we imaged post-lesion sprouting of cerebellar climbing fibres (CFs) in mice using in vivo time-lapse microscopy. Here we show that newly sprouted CF collaterals innervate multiple Purkinje cells (PCs) over several months, with most innervations emerging at 3–4 weeks post lesion. Simultaneous imaging of cerebellar functional structure reveals that surviving CFs similarly innervate functionally relevant and non-relevant PCs, but have more synaptic area on PCs near the collateral origin than on distant PCs. These results suggest that newly sprouted axon collaterals do not preferentially innervate functionally relevant postsynaptic targets. Nonetheless, the spatial gradient of collateral innervation might help to loosely maintain functional synaptic circuits if functionally relevant neurons are clustered in the lesioned area. Neurodegenerative lesions induce sprouting from surviving axons, but the patterns of re-innervation of these collaterals in relation to existing functional networks remains unclear. Here the authors performed long term in vivo imaging in mice, of sprouts from cerebellar climbing fibers after a lesion, and describe the patterns of connectivity relative to functionally active zones.
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影响因子：
16.2
作者：
Nishiyama, Hiroshi;Fukaya, Masahiro;Linden, David J.
通讯作者：
Linden, David J.
影响因子：
2.5
作者：
ROSSI, F;VANDERWANT, JJL;STRATA, P
通讯作者：
STRATA, P
影响因子：
2.5
作者：
ROSSI, F;WIKLUND, L;STRATA, P
通讯作者：
STRATA, P
DOI：
10.1523/jneurosci.4561-12.2013
发表时间：
2013-05-01
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Carrillo J;Nishiyama N;Nishiyama H
通讯作者：
Nishiyama H
影响因子：
14.5
作者：
CARR, LJ;HARRISON, LM;STEPHENS, JA
通讯作者：
STEPHENS, JA