Consensus M2e peptide conjugated to gold nanoparticles confers protection against H1N1, H3N2 and H5N1 influenza A viruses.
Consensus M2e peptide conjugated to gold nanoparticles confers protection against H1N1, H3N2 and H5N1 influenza A viruses.
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共有的M2E肽与金纳米颗粒结合的肽赋予了针对H1N1，H3N2和H5N1流感病毒的保护。
DOI：
10.1016/j.antiviral.2017.01.021
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发表时间：
2017-05
影响因子：
7.6
通讯作者：
Gill HS
中科院分区：
文献类型：
--
作者：
Tao W;Hurst BL;Shakya AK;Uddin MJ;Ingrole RS;Hernandez-Sanabria M;Arya RP;Bimler L;Paust S;Tarbet EB;Gill HS
The extracellular domain of influenza A ion channel membrane matrix protein 2 (M2e) is considered to be a potential candidate to develop a universal influenza A vaccine. However poor immunogenicity of M2e presents a significant roadblock. We have developed a vaccine formulation comprising of the consensus M2e peptide conjugated to gold nanoparticles (AuNPs) with CpG as a soluble adjuvant (AuNP-M2e+sCpG). We demonstrate that intranasal delivery of AuNP-M2e+sCpG in mice induces lung B cell activation and robust serum anti-M2e immunoglobulin G (IgG) response, with stimulation of both IgG1 and IgG2a subtypes. Using Madin-Darby canine kidney (MDCK) cells infected with A/California/04/2009 (H1N1pdm) pandemic strain, or A/Victoria/3/75 (H3N2), or the highly pathogenic avian influenza virus A/Vietnam/1203/2004 (H5N1) as immunosorbants we further show that the antibodies generated are also capable of binding to the homotetrameric form of M2 expressed on infected cells. Lethal challenge of vaccinated mice with A/California/04/2009 (H1N1pdm) pandemic strain, A/Victoria/3/75 (H3N2), and the highly pathogenic avian influenza virus A/Vietnam/1203/2004 (H5N1) led to 100%, 92%, and 100% protection, respectively. Overall, this study helps to lay the foundation of a potential universal influenza A vaccine.
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影响因子：
5.5
作者：
Kim, Min-Chul;Lee, Yu-Na;Hwang, Hye Suk;Lee, Young-Tae;Ko, Eun-Ju;Jung, Yu-Jin;Cho, Min Kyoung;Kim, Yu-Jin;Lee, Jong Seok;Ha, Suk-Hoon;Kang, Sang-Moo
通讯作者：
Kang, Sang-Moo
DOI：
10.4049/jimmunol.0801814
发表时间：
2009-03-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hamada H;Garcia-Hernandez Mde L;Reome JB;Misra SK;Strutt TM;McKinstry KK;Cooper AM;Swain SL;Dutton RW
通讯作者：
Dutton RW
DOI：
10.1073/pnas.94.18.9814
发表时间：
1997-09-02
影响因子：
11.1
作者：
Elkon, KB;Liu, CC;FalckPedersen, E
通讯作者：
FalckPedersen, E
影响因子：
0.8
作者：
Ingrole, Rohan S.;Tao, Wenqian;Gill, Harvinder S.
通讯作者：
Gill, Harvinder S.
影响因子：
2.8
作者：
Kaufmann, A;Salentin, R;Sprenger, H
通讯作者：
Sprenger, H