Overexpression of ZmNF-YC13 Confers ER Stress Tolerance in Maize
Overexpression of ZmNF-YC13 Confers ER Stress Tolerance in Maize
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ZmNF-YC13 的过表达赋予玉米 ER 胁迫耐受性
DOI：
10.1007/s12374-021-09307-4
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发表时间：
2021-03
影响因子：
2.9
通讯作者：
Yilin Cai
中科院分区：
文献类型：
--
作者：
Xiupeng Mei;Chaoxian Liu;Jin Nan;Zikun Zhao;Yang Bai;Erfei Dong;Yilin Cai
未折叠或错误折叠的蛋白质在内质网（ER）腔中的过度积累可触发ER应激。虽然调节植物的这种胁迫反应是减轻胁迫造成的损害的重要而有效的手段，但玉米中相关的基因和机制在很大程度上仍然未知。在这项研究中，我们确定了ER应激反应基因ZmNF-YC 13，它编码一种定位于玉米细胞核和细胞质中的蛋白质。发现ZmNF-YC 13在玉米和拟南芥中的过表达赋予植物ER胁迫耐受性，并且基因表达分析揭示该基因的过表达上调参与蛋白质折叠和ER相关降解（ERAD）系统的基因的表达，包括ERdj、BiP和E3泛素连接酶。此外，我们确定ZmNF-YC 13激活原生质体中伴侣基因ZmERdj和ZmBiP的启动子，其在消除ER中未折叠和错误折叠的蛋白质中起作用。基于我们的研究结果，我们认为ZmNF-YC 13通过控制伴侣基因正向调节玉米内质网应激反应，我们相信这些结果可以为进一步的玉米抗性育种提供有价值的信息。
Excessive accumulation of unfolded or misfolded proteins in the lumen of the endoplasmic reticulum (ER) can trigger ER stress. Although regulation of this stress response in plants is an important and effective means of mitigating the damage caused by stress, the associated genes and mechanisms in maize remain largely unknown. In this study, we identified the ER stress-responsive gene ZmNF-YC13, which encodes a protein localized in the nuclei and cytoplasm of maize cells. The overexpression of ZmNF-YC13 in maize and Arabidopsis was found to confer plant ER stress tolerance, and gene expression analyses revealed that the overexpression of this gene upregulates the expression of genes involved in protein folding and the ER-associated degradation (ERAD) system, including ERdj, BiP, and E3 ubiquitin ligase. Moreover, we established that ZmNF-YC13 activates the promoters of the chaperone genes ZmERdj and ZmBiP in protoplasts, which function in the elimination of unfolded and misfolded proteins in the ER. Based on our findings, we propose that ZmNF-YC13 positively regulates the ER stress response in maize by controlling chaperone genes, and we believe that these results could provide valuable information for further maize resistance breeding.
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影响因子：
3.3
作者：
Shen, Y;Hendershot, LM
通讯作者：
Hendershot, LM
影响因子：
6.9
作者：
Yadav D;Shavrukov Y;Bazanova N;Chirkova L;Borisjuk N;Kovalchuk N;Ismagul A;Parent B;Langridge P;Hrmova M;Lopato S
通讯作者：
Lopato S
影响因子：
2.9
作者：
Jin, Yi;Zhuang, Min;Hendershot, Linda M.
通讯作者：
Hendershot, Linda M.
影响因子：
4.9
作者：
Takahashi, Hideyuki;Kawakatsu, Taiji;Takaiwa, Fumio
通讯作者：
Takaiwa, Fumio
DOI：
10.1073/pnas.0408941102
发表时间：
2005-04-05
影响因子：
11.1
作者：
Iwata, Y;Koizumi, N
通讯作者：
Koizumi, N