Evaluating Ketosis in Primate Field Studies: Validation of Urine Test Strips in Wild Bornean Orangutans (Pongo pygmaeus wurmbii)
Evaluating Ketosis in Primate Field Studies: Validation of Urine Test Strips in Wild Bornean Orangutans (Pongo pygmaeus wurmbii)
复制标题
评估灵长类动物实地研究中的酮症：野生婆罗洲猩猩 (Pongo pygmaeus wurmbii) 尿液试纸条的验证
DOI：
10.1159/000501933
复制
发表时间：
2020
影响因子：
1.9
通讯作者：
Vogel, Erin R.
中科院分区：
文献类型：
--
作者：
Naumenko, Daniel J.;Watford, Malcolm;Utami Atmoko, Sri Suci;Erb, Wendy M.;Vogel, Erin R.
尿液测试条的使用（例如，Roche Chemstrip®）已成为快速评估野生灵长类动物生理状况和/或健康的标准。这些条已被用来检测酮症作为脂肪catalysts在几个灵长类动物类群在其自然环境中，以应对不断变化的食物供应的标记。然而，使用尿试纸测定酮症仅在人类研究中得到验证，因此尚不清楚这些试纸是否能准确检测和定量非人灵长类动物中的酮体。我们研究了从端南猩猩研究站收集的野生婆罗洲猩猩尿液样本中酮体浓度的变化。我们在现场评估了来自Chemstrip测试条的定性结果的准确性（即，阴性、小、中等和大），在实验室中使用酶联测定法测定相同尿样的乙酰乙酸盐浓度。与检测阴性的尿液样本相比，现场检测酮阳性的尿液样本在酶法检测中的酮水平显著较高。在4个Chemstrip值中，乙酰乙酸盐浓度存在显著差异;然而，事后检验显示阴性样本和小样本之间无显著差异。我们的结论是，尿试纸提供了一个有用的工具，用于确定野生猩猩的酮症状态，但应谨慎解释这些条上仅显示少量酮的样品的结果时。
The use of urine test strips (e.g., Roche Chemstrip®) has become the standard for quickly assessing the physiological condition and/or health of wild primates. These strips have been used to detect ketosis as a marker of fat catabolism in several primate taxa in their natural environments in response to changing food availability. However, the use of urine strips to determine ketosis has only been validated in human studies, and thus it remains unclear whether these strips accurately detect and quantify ketone bodies in nonhuman primates. We examined variations in ketone body concentrations in urine samples collected from wild Bornean orangutans at the Tuanan Orangutan Research Station. We assessed the accuracy of qualitative results from Chemstrip test strips in the field (i.e., negative, small, moderate, and large) using an enzyme-linked assay in the laboratory to determine the concentrations of acetoacetate of the same urine samples. Urine samples that tested positive for ketones in the field had significantly higher levels of ketones in the enzymatic assay compared to those that tested negative. There was significant variation in acetoacetate concentrations among the 4 Chemstrip values; however, post hoc tests revealed no significant differences between negative and small samples. We conclude that urinary test strips provide a useful tool for determining ketotic state in wild orangutans, but caution should be taken when interpreting results from samples showing only small levels of ketones on these strips.
登录
查看更多内容
野生白手长臂猿（Hylobates lar）种群中的不育脓尿
影响因子：
2.4
作者：
Barbara A. Beaman;Wyatt J. Hesemeyer;N. Dominy;T. Savini;U. Reichard
通讯作者：
U. Reichard
影响因子：
2.5
作者：
Harrison, Mark E.;Morrogh-Bernard, Helen C.;Chivers, David J.
通讯作者：
Chivers, David J.
社会地位和压力对野生白掌长臂猿（Hylobates lar）胃肠道寄生模式的影响。
影响因子：
2.8
作者：
T. Gillespie;C. Barelli;M. Heistermann
通讯作者：
M. Heistermann
胰岛素尿 C 肽作为野生猩猩能量平衡的非侵入性标志物
影响因子：
3.5
作者：
M. E. Thompson;C. Knott
通讯作者：
C. Knott
影响因子：
2.5
作者：
Krief, S;Huffman, MA;Wrangham, RW
通讯作者：
Wrangham, RW