A Sensitive Electrochemical Immunosensor Based on PAMAM Dendrimer-Encapsulated Au for Detection of Norfloxacin in Animal-Derived Foods.
A Sensitive Electrochemical Immunosensor Based on PAMAM Dendrimer-Encapsulated Au for Detection of Norfloxacin in Animal-Derived Foods.
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DOI：
10.3390/s18061946
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发表时间：
2018-06-15
期刊：
影响因子：
--
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Liu B;Li M;Zhao Y;Pan M;Gu Y;Sheng W;Fang G;Wang S
本文报道了一种灵敏的电化学免疫传感器用于动物源性食品中诺氟沙星的检测。聚酰胺-胺树枝状大分子包埋的金纳米粒子（PAMAM-Au）在该传感平台中起到了双重作用，不仅加速了传感的电子传递过程，而且提高了固定化抗体的效率。HRP标记的抗原作为免疫传感器中的信号标记物，在竞争反应中，在H2 O2的辅助下催化底物对苯二酚的后续反应。在PAMAM-Au的信号放大作用的基础上，诺氟沙星的浓度在1 μg·L-1-10 mg·L-1范围内与信号强度呈线性关系。结果表明，该方法检出限低（0.3837 μg·L-1），回收率在91.6%~ 106.1%之间，灵敏度高、准确度好、稳定性好，可用于动物源性食品中诺氟沙星的检测。
In this work, a sensitive electrochemical immunosensor has been reported for the determination of norfloxacin in animal-derived foods. The poly (amidoamine) dendrimer encapsulated gold nanoparticles (PAMAM-Au) played dual roles in the proposed sensing platform which not only accelerated the electron transfer process of sensing, but also increased the efficiency of the immobilized antibody. The HRP-labeled antigen, as the signal labels in the immunosensor, was introduced to catalyze the following reaction of the substrate hydroquinone with the aid of H2O2 in the competitive reaction. On the basis of the signal amplification of PAMAM-Au, the signal intensity was linearly related to the concentration of norfloxacin in the range of 1 μg·L−1–10 mg·L−1. All the results showed that the proposed strategy with low LOD (0.3837 μg·L−1) and favorable recovery (91.6–106.1%) in the practical sample, and it could provide a suitable protocol for norfloxacin detection in animal-derived foods with high sensitivity, good accuracy, and stability.
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