Acceleration of spinal fusion using syngeneic and allogeneic adult adipose derived stem cells in a rat model.
Acceleration of spinal fusion using syngeneic and allogeneic adult adipose derived stem cells in a rat model.
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在大鼠模型中，使用同种异体和同种异体成年脂肪衍生的干细胞加速脊柱融合。
DOI：
10.1002/jor.20735
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发表时间：
2009-03
影响因子：
2.8
通讯作者：
Gimble, Jeffrey M.
中科院分区：
文献类型：
--
作者：
Lopez, Mandi J.;McIntosh, Kevin R.;Spencer, Nakia D.;Borneman, Jade N.;Horswell, Ronald;Anderson, Paul;Yu, Gang;Gaschen, Lorrie;Gimble, Jeffrey M.
后外侧脊柱融合术是腰椎压缩性骨折的标准治疗方法。成人脂肪组织来源的干细胞（ASCs）在体内和体外促进成骨。本研究检验的假设是，在由磷酸三钙和胶原组成的生物材料支架上的同基因和异基因ASCs将加速大鼠模型的脊柱融合。将雄性Fischer大鼠或ACI大鼠的ASCs装载到支架上（53,571个细胞/mm3），并在基质培养基中培养48小时。雄性Fisher大鼠在双侧L4和L5横突去皮后分为4个队列（n = 14/队列）：(1)不处理；(2)仅脚手架；(3)支架+同源ASCs；(4)支架+异体造血干细胞。每个队列中有一半在手术后4或8周切除。通过x线片、显微计算机断层扫描和光学显微镜评估脊柱融合。没有支架的脊柱不形成愈伤组织。术后4周支架组间骨痂形成无显著差异。术后8周，基于显微结构以及活跃骨形成的x线摄影和显微计算机断层摄影证据，ASC组和支架组的骨痂形成比单独支架组的骨痂形成更成熟。术后4周，两个ASC组（同基因组= 18.3±0.85%；异基因组= 23.5±2.33%）的炎症细胞浸润明显低于支架组（46.8±11.8%）。本研究的结果支持在大鼠模型中同基因和异体ASC加速后路腰椎融合。
Posterolateral spinal fusion is the standard treatment for lumbar compression fractures. Adult adipose tissue-derived stem cells (ASCs) promote osteogenesis in vivo and in vitro. The hypothesis tested in this study was that syngeneic and allogeneic ASCs on a biomaterial scaffold composed of tricalcium phosphate and collagen I will accelerate spinal fusion in a rat model. ASCs from male Fischer or ACI rats were loaded onto scaffolds (53,571 cells/mm3) and cultured in stromal media for 48 h. Male Fisher rats were assigned to 4 cohorts (n = 14/cohort) after bilateral decortication of the L4 and L5 transverse processes: (1) No treatment; (2) scaffold only; (3) scaffold + syngeneic ASCs; or (4) scaffold + allogeneic ASCs. Half of each cohort was harvested 4 or 8 weeks after surgery. Spinal fusion was evaluated with radiographs, microcomputed tomography, and light microscopy. Callus did not form in spines without scaffolds. There were no significant differences in callus formation among scaffold cohorts 4 weeks after surgery. Callus formation was more mature in both ASC cohorts versus scaffold alone 8 weeks after surgery based on microstructure as well as radiographic and microcomputed tomographic evidence of active bone formation. Inflammatory cell infiltrate was significantly lower in both ASC cohorts (syngeneic = 18.3±0.85%; allogeneic = 23.5±2.33%) versus scaffold alone (46.8±11.8%) 4 weeks after surgery. Results of this study support syngeneic and allogeneic ASC acceleration of posterior lumbar spinal fusion in a rat model.
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影响因子：
2.6
作者：
Mankani, Mahesh H.;Kuznetsov, Sergei A.;Robey, Pamela Gehron
通讯作者：
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