Spontaneous synaptic activity in an organotypic culture of the mouse retina.
Spontaneous synaptic activity in an organotypic culture of the mouse retina.
复制标题
小鼠视网膜器官型培养物中的自发突触活动。
DOI：
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发表时间：
2003
影响因子：
4.4
通讯作者：
K. H. Backus
中科院分区：
文献类型：
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作者：
J. Pérez;M. Frech;Jörn E. Schröder;Frauke Fischer;M. Kneussel;H. Wässle;K. H. Backus
目的
许多品系的突变小鼠在出生时就会死亡，当时视网膜还很不成熟。这类小鼠的视网膜可以在器官培养中进行研究。在对突触发育进行了先前的解剖学研究之后，我们在野生型和吉普林缺乏的小鼠身上研究了这些培养物中突触回路的电活动。
方法
体外培养新生小鼠视网膜器官类型培养14天。采用膜片钳全细胞记录技术测定无长突细胞自发突触后电流(SPSCs)。比较了用特异性拮抗剂分离的GABA能和甘氨酸能电流，以及野生型(geph型(+/+))和吉菲林缺陷型(geph型(-/-))小鼠的视网膜。
结果
被犬尿酸阻断的快速衰退的sPSCs是由离子型谷氨酸受体介导的，而峰值明显较高且慢衰减动力学的sPSCs是由γ-氨基丁酸A型受体(GABA(A)Rs)和甘氨酸受体(Glyr)介导的自发性抑制性突触后电流(SIPSCs)。在缺乏Gen(-/-)的培养中，我们没有发现GlyRs介导的sIPSCs。GABA(A)受体介导的sIPSCs在视网膜无长突细胞中表达的速度明显快于GABA能的sIPSCs在无长突细胞中的表达。
结论
Gep(+/+)和Gep(-/-)视网膜无长突细胞表达的GABA(A)R的不同衰变动力学表明，这些细胞至少表达两种类型的GABA(A)R亚型。在gave(-/-)小鼠的无长突细胞中，一种特定的GABA(A)R亚型可能包含α2亚单位，由于缺乏Gen，该亚型受到损害，而其他GABA(A)R似乎功能正常。
PURPOSE
Many strains of mutant mice die at birth, when the retina is still very immature. The retinas of such mice can be studied in organotypic cultures. After a preceding anatomic study of the synaptic development, the electrical activity of the synaptic circuits within such cultures was studied in wild-type and gephyrin-deficient mice.
METHODS
Organotypic cultures of newborn mouse retinas were grown for 14 days in vitro. Spontaneous postsynaptic currents (sPSCs) of amacrine cells were measured by using the whole-cell configuration of the patch-clamp technique. GABAergic and glycinergic currents that were isolated with specific antagonists, and retinas from wild-type (geph(+/+)) and gephyrin-deficient (geph(-/-)) mice were compared.
RESULTS
Rapidly decaying sPSCs that were blocked by kynurenic acid were mediated by ionotropic glutamate receptors, whereas sPSCs with significantly higher peak amplitudes and slow-decay kinetics were identified as spontaneous inhibitory postsynaptic currents (sIPSCs) mediated by gamma-aminobutyric acid type A receptors (GABA(A)Rs) and glycine receptors (GlyRs). In gephyrin-deficient (geph(-/-)) cultures, we found no sIPSCs mediated by GlyRs. sIPSCs mediated by GABA(A)Rs expressed in amacrine cells of geph(-/-) retinas decayed significantly faster than GABAergic sIPSCs recorded in amacrine cells of geph(+/+) retinas.
CONCLUSIONS
The different decay kinetics of GABA(A)Rs expressed in amacrine cells of geph(+/+) and of geph(-/-) retinas suggests that these cells express at least two types of GABA(A)R subtypes. In amacrine cells of geph(-/-) mice, a specific GABA(A)R subtype that may contain the alpha2 subunit, is impaired by the absence of gephyrin, whereas other GABA(A)Rs appear to function normally.
培养兔视网膜外植体中 GABA 能表型的表达和下调。
影响因子：
4.4
作者：
Rowe-Rendleman,C;Mitchell,CK;Habrecht,M;Redburn,DA
通讯作者：
Redburn,DA
影响因子：
3.4
作者：
Lavoie, AM;Tingey, JJ;Twyman, RE
通讯作者：
Twyman, RE
影响因子：
56.9
作者：
MEISTER, M;WONG, ROL;SHATZ, CJ
通讯作者：
SHATZ, CJ