Increased levels of IAA are required for system 2 ethylene synthesis causing fruit softening in peach (Prunus persica L. Batsch).
Increased levels of IAA are required for system 2 ethylene synthesis causing fruit softening in peach (Prunus persica L. Batsch).
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DOI：
10.1093/jxb/ers381
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发表时间：
2013-02
影响因子：
6.9
通讯作者：
Nakamura Y
中科院分区：
文献类型：
--
作者：
Tatsuki M;Nakajima N;Fujii H;Shimada T;Nakano M;Hayashi K;Hayama H;Yoshioka H;Nakamura Y
以桃（Prunus persica L.）Batsch）品种产生高水平的乙烯，这是由PpACS 1（1-氨基环丙烷-1-羧酸合酶的同功基因）的高表达引起的，导致果实在后熟阶段快速软化。相反，由于PpACS 1的低表达，石质硬桃品种的果实不软化，产生很少的乙烯。为了阐明抑制PpACS 1在多石硬桃中表达的机制，进行了微阵列分析。鉴定了几个表现出与PpACS 1相似表达模式的基因，并显示为吲哚-3-乙酸（IAA）诱导基因（Aux/IAA，SAUR）。也就是说，IAA诱导基因的表达增加，在成熟后期阶段，在融化的肉桃，然而，这些成绩单是低的石头硬桃成熟果实。IAA浓度突然增加，正好与系统2乙烯的生产在融化的肉桃收获前的时间。与此相反，IAA浓度并没有增加，在石硬桃。应用1-萘乙酸，一种合成的生长素，对石硬桃诱导高水平的PpACS 1表达，大量的乙烯产生和软化。将抗生长素α-（苯乙基-2-酮）-IAA应用于融化的果肉桃，降低了PpACS 1表达和乙烯产生的水平。这些观察结果表明，抑制PpACS 1的表达在晚熟阶段的石硬桃可能会导致低水平的IAA和高浓度的IAA是需要产生大量的系统2乙烯在桃。
The fruit of melting-flesh peach (Prunus persica L. Batsch) cultivars produce high levels of ethylene caused by high expression of PpACS1 (an isogene of 1-aminocyclopropane-1-carboxylic acid synthase), resulting in rapid fruit softening at the late-ripening stage. In contrast, the fruit of stony hard peach cultivars do not soften and produce little ethylene due to low expression of PpACS1. To elucidate the mechanism for suppressing PpACS1 expression in stony hard peaches, a microarray analysis was performed. Several genes that displayed similar expression patterns as PpACS1 were identified and shown to be indole-3-acetic acid (IAA)-inducible genes (Aux/IAA, SAUR). That is, expression of IAA-inducible genes increased at the late-ripening stage in melting flesh peaches; however, these transcripts were low in mature fruit of stony hard peaches. The IAA concentration increased suddenly just before harvest time in melting flesh peaches exactly coinciding with system 2 ethylene production. In contrast, the IAA concentration did not increase in stony hard peaches. Application of 1-naphthalene acetic acid, a synthetic auxin, to stony hard peaches induced a high level of PpACS1 expression, a large amount of ethylene production and softening. Application of an anti-auxin, α-(phenylethyl-2-one)-IAA, to melting flesh peaches reduced levels of PpACS1 expression and ethylene production. These observations indicate that suppression of PpACS1 expression at the late-ripening stage of stony hard peach may result from a low level of IAA and that a high concentration of IAA is required to generate a large amount of system 2 ethylene in peaches.
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影响因子：
4.3
作者：
Haji, T;Yaegaki, H;Yamaguchi, M
通讯作者：
Yamaguchi, M
影响因子：
7
作者：
Begheldo, Maura;Manganaris, George A.;Tonutti, Pietro
通讯作者：
Tonutti, Pietro
DOI：
10.1073/pnas.76.1.170
发表时间：
1979-01-01
影响因子：
11.1
作者：
ADAMS, DO;YANG, SF
通讯作者：
YANG, SF
影响因子：
6.9
作者：
Tatsuki, M;Haji, T;Yamaguchi, M
通讯作者：
Yamaguchi, M
DOI：
10.2503/jjshs.70.458
发表时间：
2001-07-01
影响因子：
--
作者：
Haji, T;Yaegaki, H;Yamaguchi, M
通讯作者：
Yamaguchi, M