Prevalence and Characterization of Staphylococcus aureus Isolated From Women and Children in Guangzhou, China.
Prevalence and Characterization of Staphylococcus aureus Isolated From Women and Children in Guangzhou, China.
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DOI：
10.3389/fmicb.2018.02790
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发表时间：
2018
影响因子：
5.2
通讯作者：
Zhou Z
中科院分区：
文献类型：
--
作者：
Liang B;Mai J;Liu Y;Huang Y;Zhong H;Xie Y;Deng Q;Huang L;Yao S;He Y;Long Y;Yang Y;Gong S;Yang H;Zhou Z
众所周知，流行的金黄色葡萄球菌克隆和抗生素敏感性谱会发生动态和地理变化；然而，最近引起中国妇女和儿童感染的金黄色葡萄球菌菌株的特征尚未确定。在这项研究中，我们分析了从中国广州四个妇女儿童中心患者分离出的金黄色葡萄球菌的分子流行病学和抗菌药物耐药性。通过 spa 分型、多位点序列分型、毒力基因和抗菌素耐药性分析、葡萄球菌染色体盒 mec 分型以及 rpoB 突变分析，总共分析了 131 株金黄色葡萄球菌分离株（100 株来自儿童，31 株来自女性）。总共鉴定了 58 种 spa 类型、27 种序列类型 (ST) 和 10 种克隆复合物 (CC)。 CC59 (ST59-IV, 48.8%; ST338-III, 35.7%) 和 CC45 (ST45-IV, 100%) 是 MRSA 分离株中的主要克隆 (84.4%)，而 CC5 (ST188, 24.3%; ST1, 21.6%) 和 CC398 (ST398, 70%) 是主要克隆(70.1%) MSSA 分离株。 ST338-MRSA-III主要存在于脓液中，但在呼吸道样本中很少见，而ST45-MRSA-IV则相反，尽管它们都经常在血液和脑脊液样本中发现。葡萄球菌肠毒素基因seb-seq-sek与ST59和ST338密切相关，而sec与ST45、ST121、ST22和ST30相关。所有 ST338、ST1232 和 SCCmec III 分离株均携带 lukF/S-PV 基因。总共 80% 的 ST338 分离株对红霉素、克林霉素和四环素具有耐药性。所有 ST45 分离株均表现出对利福平的中度或完全耐药性。在利福平耐药或中度耐药菌株中总共发现了 481 个 rpoB 中的 HIS/ASN 突变。 ST338-III 和 ST45-IV 是中国广州妇女和儿童 MRSA 分离株中三个主要克隆中的两个，但 ST59-MRSA-IV 仍然是最常见的 MRSA 克隆。金黄色葡萄球菌的克隆分布因样本来源而异。毒力基因和抗菌谱与克隆谱系密切相关。这些结果阐明了中国广州地区妇女和儿童金黄色葡萄球菌的分子流行病学，为控制和治疗金黄色葡萄球菌感染提供了重要信息。
The prevalent Staphylococcus aureus clones and antibiotic susceptibility profiles are known to change dynamically and geographically; however, recent S. aureus strains causing infections in women and children in China have not been characterized. In this study, we analyzed the molecular epidemiology and antimicrobial resistance of S. aureus isolated from patients in four centers for women and children in Guangzhou, China. In total, 131 S. aureus isolates (100 from children and 31 from women) were analyzed by spa typing, multi-locus sequence typing, virulence gene and antimicrobial resistance profiling, staphylococcal chromosomal cassette mec typing, and mutation analyses of rpoB. A total of 58 spa types, 27 sequence types (STs), and 10 clonal complexes (CCs) were identified. While CC59 (ST59-IV, 48.8%; ST338-III, 35.7%) and CC45 (ST45-IV, 100%) were the major clones (84.4%) among MRSA isolates, CC5 (ST188, 24.3%; ST1, 21.6%) and CC398 (ST398, 70%) were the major ones (70.1%) among MSSA isolates. ST338-MRSA-III mostly found in pus but hardly in respiratory tract samples while ST45-MRSA-IV was on the opposite, even though they both found in blood and cerebrospinal fluid sample frequently. Staphylococcal enterotoxin genes seb-seq-sek were strongly associated with ST59 and ST338, while sec was associated with ST45, ST121, ST22, and ST30. All ST338, ST1232, and SCCmec III isolates carried lukF/S-PV genes. A total of 80% of ST338 isolates were resistant to erythromycin, clindamycin, and tetracycline. All ST45 isolates exhibited intermediate or complete resistance to rifampicin. In total, 481 HIS/ASN mutations in rpoB were found in rifampicin-resistant or intermediate-resistant isolates. ST338-III and ST45-IV emerged as two of three major clones in MRSA isolates from women and children in Guangzhou, China, though ST59-MRSA-IV remained the most prevalent MRSA clone. Clonal distribution of S. aureus varied, depending on the specimen source. Virulence genes and antibiograms were closely associated with the clonal lineage. These results clarified the molecular epidemiology of S. aureus from women and children in Guangzhou, China, and provide critical information for the control and treatment of S. aureus infections.
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影响因子：
3.2
作者：
Lindsay, JA;Moore, CE;Hinds, J
通讯作者：
Hinds, J
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影响因子：
5.2
作者：
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通讯作者：
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11.8
作者：
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通讯作者：
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影响因子：
3.2
作者：
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通讯作者：
Spratt, BG
影响因子：
4.8
作者：
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