Two neutrophil populations in human blood with different chemotactic activities: separation and chemoattractant binding
Two neutrophil populations in human blood with different chemotactic activities: separation and chemoattractant binding
复制标题
人血液中具有不同趋化活性的两个中性粒细胞群：分离和趋化剂结合
作者：
L. Harvath;E. Leonard
正常人血液中含有一群嗜中性粒细胞，它们迁移到各种化学引诱物中，还有一群不能迁移。中性粒细胞迁移至最佳浓度的化学引诱物的百分比被定量：20 - 40%迁移至N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸，30 - 50%迁移至人C5 a，25 - 35%迁移至人白细胞衍生的趋化因子，20 - 30%迁移至酪蛋白，15 - 20%迁移至胃酶抑制剂，1 - 5%迁移至单独的培养基。当加入其他化学引诱物时，中性粒细胞迁移到最活跃的化学引诱物没有增加，表明迁移到最活跃的引诱物的中性粒细胞群体与迁移到其他引诱物的群体相同。通过延长试验孵育期或在试验期间用新的化学引诱物替换引诱物，中性粒细胞迁移至化学引诱物的百分比没有改变，并且该百分比与加入趋化室的中性粒细胞浓度无关。用趋化性收集室分离非迁移性中性粒细胞，并检查它们是否与放射性标记的趋化肽结合。非迁移性和迁移性中性粒细胞与放射性标记的N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸的结合是相同的。
Normal human blood contains a population of neutrophils that migrates to various chemoattractants and a population that fails to migrate. The percentage of neutrophils migrating to optimal concentrations of chemoattractants was quantified: 20 to 40% migrated to N-formylmethionyl-leucyl-phenylalanine, 30 to 50% migrated to human C5a, 25 to 35% migrated to human leukocyte-derived chemotactic factors, 20 to 30% migrated to casein, 15 to 20% migrated to pepstatin, and 1 to 5% migrated to medium alone. Neutrophil migration to the most active chemoattractant was not increased when other chemoattractants were added, indicating that the population of neutrophils migrating to the most active attractant was the same population that was migrating to the other attractants. The percentage of neutrophils migrating to a chemoattractant was not altered by prolonging the assay incubation period or by replacing the attractant with new chemoattractant during the assay, and the percentage was independent of the neutrophil concentration added to the chemotaxis chamber. Nonmigrating neutrophils were isolated with a chemotaxis collection chamber, and they were examined for radiolabeled chemotactic peptide binding. The binding of radiolabeled N-formylmethionyl-leucyl-phenylalanine by nonmigrating and migrating neutrophils was identical.
单核细胞对趋化刺激的反应性是可以对多种趋化剂作出反应的细胞亚群的特性。
DOI：
10.1172/jci110033
发表时间：
1981
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Cianciolo,GJ;Snyderman,R
通讯作者：
Snyderman,R