Postnatal depletion of maternal cells biases T lymphocytes and natural killer cells' profiles toward early activation in the spleen.
Postnatal depletion of maternal cells biases T lymphocytes and natural killer cells' profiles toward early activation in the spleen.
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在妊娠期间转移到胎儿体内的母体细胞在后代出生后很长时间内仍然存在。这些母体细胞被假设为促进子宫内胎儿免疫系统的成熟，但我们对它们在出生后的潜在作用的认识仍然存在重大差距。为了深入了解这些母体细胞的出生后功能作用，我们建立了一个转基因小鼠模型，通过注射白喉毒素特异性消除新生儿中的母体细胞，并证实了脾脏中的显著耗竭。然后，我们通过质谱细胞术对两周龄幼鼠的脾脏进行免疫表型分析，以确定出生后早期母体细胞耗竭导致的免疫特征差异。我们观察到在一些自然杀伤细胞和T细胞群体中与激活和成熟相关的标志物的表达增加。我们假设这些结果表明一个潜在的产后调节淋巴细胞反应的母体细胞。总之，我们的研究结果强调了母体微嵌合细胞出生后的免疫影响，可能保护新生儿在新的自我和环境抗原的关键时刻的不良超敏反应。总结：使用白喉毒素受体转基因小鼠特异性耗竭新生儿母体细胞后，观察到脾脏T细胞和自然杀伤细胞的早期激活。
The maternal cells transferred into the fetus during gestation persist long after birth in the progeny. These maternal cells have been hypothesized to promote the maturation of the fetal immune system in utero but there are still significant gaps in our knowledge of their potential roles after birth. To provide insights into these maternal cells’ postnatal functional roles, we set up a transgenic mouse model to specifically eliminate maternal cells in the neonates by diphtheria toxin injection and confirmed significant depletion in the spleens. We then performed immunophenotyping of the spleens of two-week-old pups by mass cytometry to pinpoint the immune profile differences driven by the depletion of maternal cells in early postnatal life. We observed a heightened expression of markers related to activation and maturation in some natural killer and T cell populations. We hypothesize these results to indicate a potential postnatal regulation of lymphocytic responses by maternal cells. Together, our findings highlight an immunological influence of maternal microchimeric cells postnatally, possibly protecting against adverse hypersensitivity reactions of the neonate at a crucial time of new encounters with self and environmental antigens. Summary: After specific depletion of maternal cells in neonates using diphtheria toxin receptor transgenic mice, early activation of splenic T and natural killer cells was observed.
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影响因子：
4.4
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