Unique patterns of transcript and miRNA expression in the South American strong voltage electric eel (Electrophorus electricus).
Unique patterns of transcript and miRNA expression in the South American strong voltage electric eel (Electrophorus electricus).
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DOI：
10.1186/s12864-015-1288-8
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发表时间：
2015-03-26
期刊：
影响因子：
4.4
通讯作者：
Samanta MP
中科院分区：
文献类型：
--
作者：
Traeger LL;Volkening JD;Moffett H;Gallant JR;Chen PH;Novina CD;Phillips GN Jr;Anand R;Wells GB;Pinch M;Güth R;Unguez GA;Albert JS;Zakon H;Sussman MR;Samanta MP
南美高压电鳗（Electrophorus electricus）以其独特的能力产生超过600伏的高压放电，在科学史上发挥了重要作用。值得注意的是，人们对它的电子器官的分子性质知之甚少。我们对E.包括八个成熟组织的转录组：脑、脊髓、肾、心脏、骨骼肌、Sachs'电器官、主电器官和Hunter'电器官。基于基因本体的基因集富集分析揭示了与跨膜转运、雄激素结合和信号传导相关的所有三个电子器官中的富集功能。这项研究也是首次对电鱼中的miRNA进行分析。鉴定了一系列具有电器官特异性表达模式的miRNA，其中包括一种在大肠杆菌所有三个电器官中高度过表达的新miRNA。电的所有这三种电器官组织也表达三种保守的miRNAs，据报道，这些miRNAs抑制哺乳动物的肌肉发育，这表明依赖于miRNAs的基因表达调控可能在从其肌肉前体指定电器官身份方面发挥重要作用。这些miRNA数据得到了另一个完整的miRNA图谱的支持，该图谱来自第二个裸形目物种的肌肉和电子器官组织。我们在E. electricus基因组和八个组织特异性基因表达谱将大大有助于未来确定编码和调控序列的研究，指定的功能，发育和进化的电器。此外，这些数据和未来的研究将通过本文提出的对电鱼中miRNA表达的第一次全面分析来了解。本文的在线版本（doi：10.1186/s12864-015-1288-8）包含补充材料，可供授权用户使用。
With its unique ability to produce high-voltage electric discharges in excess of 600 volts, the South American strong voltage electric eel (Electrophorus electricus) has played an important role in the history of science. Remarkably little is understood about the molecular nature of its electric organs. We present an in-depth analysis of the genome of E. electricus, including the transcriptomes of eight mature tissues: brain, spinal cord, kidney, heart, skeletal muscle, Sachs’ electric organ, main electric organ, and Hunter’s electric organ. A gene set enrichment analysis based on gene ontology reveals enriched functions in all three electric organs related to transmembrane transport, androgen binding, and signaling. This study also represents the first analysis of miRNA in electric fish. It identified a number of miRNAs displaying electric organ-specific expression patterns, including one novel miRNA highly over-expressed in all three electric organs of E. electricus. All three electric organ tissues also express three conserved miRNAs that have been reported to inhibit muscle development in mammals, suggesting that miRNA-dependent regulation of gene expression might play an important role in specifying an electric organ identity from its muscle precursor. These miRNA data were supported using another complete miRNA profile from muscle and electric organ tissues of a second gymnotiform species. Our work on the E. electricus genome and eight tissue-specific gene expression profiles will greatly facilitate future research on determining the coding and regulatory sequences that specify the function, development, and evolution of electric organs. Moreover, these data and future studies will be informed by the first comprehensive analysis of miRNA expression in an electric fish presented here. The online version of this article (doi:10.1186/s12864-015-1288-8) contains supplementary material, which is available to authorized users.
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影响因子：
14.9
作者：
Kozomara A;Griffiths-Jones S
通讯作者：
Griffiths-Jones S
影响因子：
1.8
作者：
HOFACKER, IL;FONTANA, W;SCHUSTER, P
通讯作者：
SCHUSTER, P
影响因子：
56.9
作者：
FICKER, E;TAGLIALATELA, M;BROWN, AM
通讯作者：
BROWN, AM
影响因子：
4
作者：
Ambros, Victor
通讯作者：
Ambros, Victor
DOI：
10.1126/science.1254432
发表时间：
2014-06-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gallant JR;Traeger LL;Volkening JD;Moffett H;Chen PH;Novina CD;Phillips GN Jr;Anand R;Wells GB;Pinch M;Güth R;Unguez GA;Albert JS;Zakon HH;Samanta MP;Sussman MR
通讯作者：
Sussman MR