Syndecan-1 expression during postnatal tooth and oral mucosa development in rats aged from two days to six weeks
Syndecan-1 expression during postnatal tooth and oral mucosa development in rats aged from two days to six weeks
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Syndecan-1 在 2 天至 6 周龄大鼠出生后牙齿和口腔粘膜发育过程中的表达
DOI：
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发表时间：
2002
影响因子：
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通讯作者：
David F. Wilson
中科院分区：
文献类型：
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作者：
Daniel D De Angelis;W. Sampson;O. Wiebkin;David F. Wilson
Syndecans是一个硫酸肝素蛋白聚糖家族，它调节影响细胞生长、增殖和形态的细胞-基质相互作用。本研究的目的是观察Syndecan-1在大鼠口腔黏膜发育上皮和正常大鼠磨牙牙周组织发育中的Malassez上皮细胞的表达变化，从牙冠发育晚期到初萌。采用免疫组织化学（Syndecan-1 N-18）和组织化学（Alcec Blue）观察2 ~ 42日龄大鼠Syndecan-1的表达变化。结果表明，在大鼠正常牙齿发育过程中，2 ~ 14日龄大鼠舌和腭基底层以上的口腔分层上皮以及发育中的磨牙成釉细胞中均可见Syndecan-1的标记或变强度染色。所有标本牙本质前层和牙本质层的组织化学染色一致。Syndecan-1标记或染色在大鼠牙周韧带中呈阴性，这可能表明Syndecan-1在正常的磨牙根发育过程中没有表达，或者需要继续工作来鉴定合适的标记。
Abstract Syndecans are a family of heparan sulphate proteoglycans that regulate cell-matrix interactions that influence cell growth, proliferation and morphology. The aim of this study was to observe changes in the expression of Syndecan-1 in the developing epithelium of the rat oral mucosa and in the epithelial cell rests of Malassez in the developing periodontium of normal rat molars, from late crown development through to early eruption. Immuno-histo-chemistry (Syndecan-1 N–18) and histochemistry (Alcec Blue) were used to observe changes in the expression of Syndecan-1 in rats aged two to 42 days. Results indicated that during normal tooth development in the rat, labelling or staining of variable intensity for Syndecan-1 was demonstrated in the stratified oral epithelium above the stratum basale in the rat tongue and palate, and in ameloblasts of the developing molar in rats aged two to 14 days. Histochemical staining of the predentine and dentine layers was consistent in all specimens. Labelling or staining for Syndecan-1 was negative in the rat periodontal ligament, which may suggest that either Syndecan-1 was not expressed during normal molar root development or that continued work is required for identification of a suitable label in rats.
影响因子：
3.2
作者：
HAYASHI, K;HAYASHI, M;BERNFIELD, M
通讯作者：
BERNFIELD, M