Expression of biologically active human tumor necrosis factor-alpha in transgenic potato plant.
Expression of biologically active human tumor necrosis factor-alpha in transgenic potato plant.
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生物活性人肿瘤坏死因子-α在转基因马铃薯植物中的表达。
DOI：
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发表时间：
2002
影响因子：
2.3
通讯作者：
T. Matsumura
中科院分区：
文献类型：
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作者：
K. Ohya;N. Itchoda;K. Ohashi;M. Onuma;C. Sugimoto;T. Matsumura
我们报道了利用农杆菌介导法将人肿瘤坏死因子-α(HuTNF-α)基因成功地插入马铃薯基因组中。HuTNF-α是一种已知的重要细胞因子，介导宿主对肿瘤和传染病的防御，也是一种免疫调节剂。为了增加外源基因产物在植物细胞中的表达，提出了构建的HuTNF-α的分子设计。Northern印迹、RT-PCR、ELISA法和Western印迹分别证实了转化子中的肿瘤坏死因子-α的转录和翻译。通过转化子提取液对小鼠L929细胞的杀伤作用，证实了具有生物活性的HuTNF-α在植物细胞中的表达。我们认为，在本研究中获得的HuTNF-α(15微克/克马铃薯植物组织)的表达水平可能足以引起与口服人乳中的肿瘤坏死因子-α类似的反应/效应。我们认为，在食用马铃薯植物中表达的肿瘤坏死因子-α在医学和兽医领域具有巨大的临床应用潜力。因此，需要充分探讨其有用性和适用性。
We report the successful insertion of the cDNA of human tumor necrosis factor-alpha (HuTNF-alpha) into the genome of potato plant species, Solanum tuberosum, using Agrobacterium tumefacience-mediated transformation. HuTNF-alpha is a known and essential cytokine mediating host defense against tumors and infectious diseases and an immunomodulating agent. To enhance the accumulation of foreign gene product expression in plant cells, the molecular design of the constructed HuTNF-alpha is presented. Transcription and translation of TNF-alpha in transformants were confirmed by Northern blot, RT-PCR, ELISA, and Western blot, respectively. Expression of the bioactive HuTNF-alpha in plant cells was confirmed by way of the cytotoxic effect of the extract obtained from the transformants against murine L929 cells. We think that the expression level of HuTNF-alpha (15 microg/g potato plant tissue) obtained in the present study may be sufficient to induce responses/effects similar to those generated by TNF-alpha in human milk administered orally. We believe that the TNF-alpha expressed in edible potato plants has tremendous potential for clinical use in the areas of medicine and veterinary science. Its usefulness and applicability, therefore, need to be fully explored.
DOI：
10.1073/pnas.93.11.5335
发表时间：
1996-05-28
影响因子：
11.1
作者：
Mason, HS;Ball, JM;Arntzen, CJ
通讯作者：
Arntzen, CJ
影响因子：
6.4
作者：
Tacket, CO;Mason, HS;Arntzen, CJ
通讯作者：
Arntzen, CJ