Vascular endothelial growth factor system in the cow oviduct: A possible involvement in the regulation of oviductal motility and embryo transport
Vascular endothelial growth factor system in the cow oviduct: A possible involvement in the regulation of oviductal motility and embryo transport
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牛输卵管中的血管内皮生长因子系统：可能参与输卵管运动和胚胎运输的调节
DOI：
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发表时间：
2005
影响因子：
2.5
通讯作者：
A. Miyamoto
中科院分区：
文献类型：
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作者：
M. Wijayagunawardane;S. Kodithuwakku;D. Yamamoto;A. Miyamoto
血管内皮生长因子（VEGF）是一种强有力的血管生成和通透性增强因子，在牛发情周期的排卵期输卵管中显示出最高的活性。输卵管的收缩活动在排卵期最强。因此，本研究的重点是VEGF可能参与调节牛输卵管中收缩-松弛相关物质的生物合成和分泌。还研究了输卵管中可能的自主VEGF系统及其内分泌调控。将第二代牛输卵管上皮细胞（BOEC）单独与VEGF（1 ng/ml）或与促黄体生成素（LH; 10 ng/ml）、雌二醇17-β（E2; 1 ng/ml）和/或孕酮（P4; 1 ng/ml）一起培养。采用二抗酶联免疫法（EIA）测定培养液中前列腺素（PGs）、内皮素-1（ET-1）和血管紧张素II（Ang II）的含量。采用真实的实时RT-PCR检测环氧化酶-2（考克斯-2）、前列腺素F合成酶（PGFS）、前列腺素E合成酶（PGES）、前内皮素原-1（prepro-ET-1）、内皮素转换酶-1（Ece-1）、血管紧张素转换酶-1（Ace-1）、VEGF及其受体的mRNA表达。结果表明，（1）VEGF剂量依赖性地刺激前列腺素E2（PGE 2）、前列腺素F2α（PGF 2 α）和ET-1的释放，但不刺激Ang II的释放。VEGF和VEGF与LH、E2和P4上调PG、ET-1生物合成级联的mRNA表达以及它们的释放。然而，只有VEGF与LH，E2和P4的组合上调Ace-1和Ang II释放的mRNA，而不是单独的VEGF。(2)LH与E2和/或P4的处理增加了VEGF、Flk-1和Flt-1的mRNA表达;（3）VEGF本身下调了VEGF的mRNA表达，LH、E2和P4增强了这种下调作用。本研究的结果提供了第一个证据，即（1）VEGF直接刺激牛输卵管中PGE 2、PGF 2 α和ET-1的生物合成和释放，（2）LH刺激输卵管VEGF系统，（3）VEGF下调输卵管VEGF系统，并且在LH存在下，这种下调进一步加强。这些数据表明，排卵前LH峰，以及格拉夫卵泡E2分泌增加和黄体（CL）退化的基础P4水平，上调输卵管VEGF系统，诱导输卵管产生最大量的收缩-松弛相关物质，用于主动输卵管收缩和配子快速运输到受精部位。然而，LH峰引起的输卵管VEGF升高似乎在排卵后立即下调输卵管VEGF系统，从而可能有助于抑制输卵管收缩，以确保胚胎在最佳时间缓慢转运至子宫。摩尔Reprod. Dev.© 2005 Wiley‐利斯公司
Vascular endothelial growth factor (VEGF) is a potent angiogenic and permeability enhancing factor, which shows the highest activity in the oviduct during the periovulatory period of the estrous cycle in cattle. It has also been shown that the contraction activity of oviduct is highest during the periovulatory period. The present study therefore focused on the possible involvement of VEGF in the regulation of biosynthesis and secretion of contraction‐relaxation‐related substances in the cow oviduct. Possible autonomous VEGF system in the oviduct as well as its endocrine control was also studied. Bovine oviductal epithelial cells (BOEC) in the second passage were cultured with VEGF (1 ng/ml) alone or with luteinizing hormone (LH; 10 ng/ml), estradiol 17‐β (E2; 1 ng/ml), and/or progesterone (P4; 1 ng/ml). The levels of prostaglandins (PGs), endothelin‐1 (ET‐1), and angiotensin II (Ang II) in the medium were measured using second antibody enzymeimmunoassay (EIA). The mRNA expressions for cycloxygenase‐2 (Cox‐2), prostaglandin F synthase (PGFS), prostaglandin E synthase (PGES), prepro‐ET‐1, endothelin converting enzyme‐1 (Ece‐1), angiotensin converting enzyme‐1 (Ace‐1), VEGF and its receptors were investigated using real‐time RT‐PCR. The results indicate that, (1) VEGF dose‐dependently stimulated the release of prostaglandin E2 (PGE2), prostaglandin F2α (PGF2α), and ET‐1, but not Ang II. VEGF and VEGF with LH, E2, and P4 upregulated mRNA expression for biosynthesis cascade of PG, ET‐1 as well as their release. However, only the combination of VEGF with LH, E2, and P4 upregulated mRNA for Ace‐1 and Ang II release, but not VEGF alone. (2) Treatments of LH, with E2 and/or P4 increased the mRNA expression for VEGF, Flk‐1 and Flt‐1, and (3) VEGF itself downregulated the expression of mRNA for VEGF, and LH, E2, and P4 enhanced this downregulatory effect. The results of the present study provide the first evidence that (1) VEGF directly stimulates the biosynthesis and release of PGE2, PGF2α, and ET‐1 in the bovine oviduct, (2) LH stimulates the oviductal VEGF system, and (3) VEGF downregulates the oviductal VEGF system and this downregulation was further intensified in the presence of LH. The data suggest that the preovulatory LH‐surge, together with increasing E2 secretion from the Graffian follicle and basal P4 levels from the regressing corpus luteum (CL), upregulates the oviductal VEGF system, inducing the maximum oviductal production of contraction–relaxation‐related substances for active oviduct contraction and rapid transport of gametes to the fertilization site. However, the oviductal VEGF elevation caused by the LH‐surge, appears to downregulate the oviductal VEGF system immediately after ovulation thereby may contribute to suppress oviductal contraction to secure slow transport of the embryo to the uterus at the optimal time. Mol. Reprod. Dev. © 2005 Wiley‐Liss, Inc.
DOI：
10.1210/jcem.81.1.8550777
发表时间：
1996
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
J. D. Gordon;S. Mesiano;C. Zaloudek;R. Jaffe
通讯作者：
J. D. Gordon;S. Mesiano;C. Zaloudek;R. Jaffe
血管内皮生长因子上调人内皮细胞中环氧合酶 2 的表达。
DOI：
10.1210/jcem.87.7.8796
发表时间：
2002
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Tamura,Mitsutoshi;Sebastian,Siby;Gurates,Bilgin;Yang,Sijun;Fang,Zongjuan;Bulun,SerdarE
通讯作者：
Bulun,SerdarE