Isolation of circulating tumor cells.
Isolation of circulating tumor cells.
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DOI：
10.1016/j.isci.2022.104696
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发表时间：
2022-08-19
期刊：
影响因子：
5.8
通讯作者：
Toner, Mehmet
中科院分区：
文献类型：
--
作者：
Edd, Jon F.;Mishra, Avanish;Smith, Kyle C.;Kapur, Ravi;Maheswaran, Shyamala;Haber, Daniel A.;Toner, Mehmet
循环肿瘤细胞（CTC）从实体瘤进入脉管系统并广泛传播以引发转移。在治疗之前、期间和之后挖掘CTC的转移富集分子特征在个性化肿瘤学中具有独特的潜力。然而，它们极其罕见，需要以高产量和纯度从大量血液中分离，但它们在大小和其他生物物理性质上与白细胞重叠。此外，许多CTC缺乏EpCAM，这是基于亲和力的捕获的基础，使其从血液中分离复杂化。本文通过分析CTC的关键隔离模式和综合隔离策略，对CTC隔离技术进行了全面的介绍。注意力集中在微流控技术的最新进展，其中正在发生加速演变的高通量分选细胞沿着多个维度。循环肿瘤细胞（CTC）通过血流传播癌症（转移）基于CTC的液体活检能够对血液中的癌细胞进行微创采样它们的极端稀有性要求从大量血液中富集所有CTC类型分析CTC分离技术，重点是高通量微流体生物技术;细胞生物学;细胞生物学的技术方面
Circulating tumor cells (CTCs) enter the vasculature from solid tumors and disseminate widely to initiate metastases. Mining the metastatic-enriched molecular signatures of CTCs before, during, and after treatment holds unique potential in personalized oncology. Their extreme rarity, however, requires isolation from large blood volumes at high yield and purity, yet they overlap leukocytes in size and other biophysical properties. Additionally, many CTCs lack EpCAM that underlies much of affinity-based capture, complicating their separation from blood. Here, we provide a comprehensive introduction of CTC isolation technology, by analyzing key separation modes and integrated isolation strategies. Attention is focused on recent progress in microfluidics, where an accelerating evolution is occurring in high-throughput sorting of cells along multiple dimensions. Circulating tumor cells (CTCs) spread cancer through the bloodstream (metastasis) CTC-based liquid biopsy enables minimally invasive sampling of cancer cells in blood Their extreme rarity requires all CTC types to be enriched from large blood volumes CTC isolation technology is analyzed, with a focus on high-throughput microfluidics Biotechnology; Cell biology; Technical aspects of cell biology
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影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
影响因子：
4.6
作者：
Fachin F;Spuhler P;Martel-Foley JM;Edd JF;Barber TA;Walsh J;Karabacak M;Pai V;Yu M;Smith K;Hwang H;Yang J;Shah S;Yarmush R;Sequist LV;Stott SL;Maheswaran S;Haber DA;Kapur R;Toner M
通讯作者：
Toner M
DOI：
10.1073/pnas.1524448113
发表时间：
2016-05-03
影响因子：
11.1
作者：
Au, Sam H.;Storey, Brian D.;Toner, Mehmet
通讯作者：
Toner, Mehmet
DOI：
10.1073/pnas.1320198111
发表时间：
2014-03-04
影响因子：
11.1
作者：
Adams, Daniel L.;Martin, Stuart S.;Cristofanilli, Massimo
通讯作者：
Cristofanilli, Massimo
影响因子：
6.1
作者：
Che J;Yu V;Garon EB;Goldman JW;Di Carlo D
通讯作者：
Di Carlo D