rBmαTX14 Increases the Life Span and Promotes the Locomotion of Caenorhabditis Elegans.
rBmαTX14 Increases the Life Span and Promotes the Locomotion of Caenorhabditis Elegans.
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rBm alpha TX14 延长寿命并促进秀丽隐杆线虫的运动
DOI：
10.1371/journal.pone.0161847
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Liu X
中科院分区：
文献类型：
--
作者：
Chen L;Zhang J;Xu J;Wan L;Teng K;Xiang J;Zhang R;Huang Z;Liu Y;Li W;Liu X
蝎子已被广泛用于各种药理学概况或作为食品供应。由于重组肽的存在，蝎毒的探索已被报道。rBmα TX 14是从东亚东亚蝎（Buthus martensii Karsch）的毒腺中提取的一种α-神经毒素，可影响离子通道电导。本文利用秀丽隐杆线虫模型研究rBmα TX 14的功能。Western blot分析表明，rBmα TX 14在大肠杆菌Rosetta（DE 3）中既表达于胞浆中，也表达于包涵体中。圆二色谱分析表明，纯化的rBmα TX 14保留了其生物学结构。用表达rBmα TX 14的大肠杆菌Rosetta（DE 3）饲喂线虫，可延长线虫的寿命，促进线虫的运动。此外，我们还鉴定了几个在C.通过微阵列分析和定量实时PCR检测线虫。此外，如果rBmα TX 14的氨基酸位点H15突变，则rBmα TX 14不再促进C. elegans寿命结果表明，rBmα TX 14在C.同时也为蝎毒重组肽的应用提供了新的研究视角。
The scorpion has been extensively used in various pharmacological profiles or as food supplies. The exploration of scorpion venom has been reported due to the presence of recombinant peptides. rBmαTX14 is an α-neurotoxin extracted from the venom gland of the East Asian scorpion Buthus martensii Karsch and can affect ion channel conductance. Here, we investigated the functions of rBmαTX14 using the Caenorhabditis elegans model. Using western blot analysis, rBmαTX14 was shown to be expressed both in the cytoplasm and inclusion bodies in the E.coli Rosetta (DE3) strain. Circular dichroism spectroscopy analysis demonstrated that purified rBmαTX14 retained its biological structures. Next, feeding nematodes with E.coli Rosetta (DE3) expressing rBmαTX14 caused extension of the life span and promoted the locomotion of the nematodes. In addition, we identified several genes that play various roles in the life span and locomotion of C. elegans through microarray analysis and quantitative real-time PCR. Furthermore, if the amino acid site H15 of rBmαTX14 was mutated, rBmαTX14 no longer promoted the C. elegans life span. In conclusion, the results not only demonstrated the functions and mechanism of rBmαTX14 in C. elegans, but also provided the new sight in the utility of recombinant peptides from scorpion venom.
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