Identification of putative orthologs of clinically relevant antimicrobial peptides in the equine ocular surface and amniotic membrane.
Identification of putative orthologs of clinically relevant antimicrobial peptides in the equine ocular surface and amniotic membrane.
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DOI：
10.1111/vop.13042
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发表时间：
2023-04
影响因子：
1.6
通讯作者：
Leonard, Brian C.
中科院分区：
文献类型：
--
作者：
Hisey, Erin A.;Martins, Bianca C.;Donnelly, Callum G.;Cassano, Jennifer M.;Katzman, Scott A.;Murphy, Christopher J.;Thomasy, Sara M.;Leonard, Brian C.
本研究旨在使用靶向 qPCR 方法和 3' 标签测序来定义马眼表面和羊膜的抗菌肽 (AMP) 表达模式。将为今后眼表先天免疫和羊膜疗法的研究提供参考。使用靶向 qPCR 方法来研究马角膜上皮、结膜和羊膜中人眼表面和羊膜中三种表达最高的 β-防御素（DEFB1、DEFB4B 和 DEFB103A）的直向同源物的存在。对来自角膜上皮、结膜和羊膜的一份样本的 RNA 进行 3' 标签测序，以进一步表征它们的 AMP 表达。马角膜上皮、结膜和羊膜表达 DEFB1、DEFB4B 和 DEFB103A。 DEFB103A 在角膜上皮中表达量最高，而 DEFB4B 在结膜和羊膜中表达量最高。来自所有三种组织的 3' 标签测序证实了这些发现，并确定了另外 5 种 β-防御素、11 种 α-防御素和两种抗菌肽的表达，其中 α-防御素显示出比 β-防御素更高的标准化读数。这项研究确定了马角膜和结膜中的 AMP 表达，表明它们在保护马眼方面发挥着关键作用，类似于人类眼表。我们还确定马羊膜表达大量 AMP，表明它可以增强作为角膜移植材料的抗菌作用。未来的研究将集中于确定这些 AMP 的抗菌活性并确定它们在微生物性角膜炎中的作用。
This study aimed to define the antimicrobial peptide (AMP) expression pattern of the equine ocular surface and amniotic membrane using a targeted qPCR approach and 3′Tag-sequencing. It will serve as a reference for future studies of ocular surface innate immunity and amniotic membrane therapies. A targeted qPCR approach was used to investigate the presence of orthologs for three of the most highly expressed beta-defensins (DEFB1, DEFB4B, and DEFB103A) of the human ocular surface and amniotic membrane in equine corneal epithelium, conjunctiva, and amniotic membrane. 3′Tag-sequencing was performed on RNA from one sample of corneal epithelium, conjunctiva, and amniotic membrane to further characterize their AMP expression. Equine corneal epithelium, conjunctiva, and amniotic membrane expressed DEFB1, DEFB4B, and DEFB103A. DEFB103A was expressed at the highest amounts in corneal epithelium, while DEFB4B was most highly expressed in conjunctiva and amniotic membrane. 3′Tag-sequencing from all three tissues confirmed these findings and identified expression of five additional beta-defensins, 11 alpha-defensins and two cathelicidins, with the alpha-defensins showing higher normalized read counts than the beta-defensins. This study identified AMP expression in the equine cornea and conjunctiva, suggesting that they play a key role in the protection of the equine eye, similar to the human ocular surface. We also determined that equine amniotic membrane expresses a substantial number of AMPs suggesting it could potentiate an antimicrobial effect as a corneal graft material. Future studies will focus on defining the antimicrobial activity of these AMPs and determining their role in microbial keratitis.
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影响因子：
2.9
作者：
MATSUZAKI, K;SUGISHITA, K;MIYAJIMA, K
通讯作者：
MIYAJIMA, K
影响因子：
4.4
作者：
Harvey, SAK;Romanowski, EG;Gordon, YJ
通讯作者：
Gordon, YJ
影响因子：
2.3
作者：
Garreis, Fabian;Schlorf, Thomas;Paulsen, Friedrich P.
通讯作者：
Paulsen, Friedrich P.
影响因子：
1.6
作者：
Lassaline, ME;Brooks, DE;Gelatt, KN
通讯作者：
Gelatt, KN
影响因子：
2.2
作者：
Lyons, Victoria N.;Townsend, Wendy M.;Liang, Siqi
通讯作者：
Liang, Siqi