Strain differences in the effect of rTMS on cortical expression of calcium-binding proteins in rats
Strain differences in the effect of rTMS on cortical expression of calcium-binding proteins in rats
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rTMS 对大鼠皮质钙结合蛋白表达影响的菌株差异
DOI：
10.1007/s00221-013-3751-6
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发表时间：
2014
影响因子：
2
通讯作者：
Funke K
中科院分区：
文献类型：
--
作者：
Benali A;Funke K
利用大鼠模型研究重复经颅磁刺激（rTMS）对皮层兴奋性变化的细胞效应，我们先前描述了连续和间歇θ-爆发刺激（cTBS，iTBS）对暗Agglutination大鼠（DA）钙结合蛋白（CaBP）小清蛋白（PV）、钙结合蛋白（CB）和钙视网膜蛋白（CR）表达的相反影响。虽然iTBS显著减少了皮质PV+细胞的数量，但不影响CB+细胞，但cTBS导致CB+细胞减少，对PV+细胞没有影响。我们的结论是，这些类别的皮质中间神经元的活动是不同的iTBS和cTBS调制。当测试另外两个大鼠品系Sprague-Dawley（SD）和Long Evans（LE）时，我们获得了偏离结果。在SD中，iTBS降低PV和CB表达，而cTBS仅降低PV表达。相比之下，重新分析的DA显示cTBS后CB表达降低，iTBS后PV表达降低，而LE显示中间反应。CR表达在任何情况下均未受影响。有趣的是，我们发现菌株的CaBP的基础表达模式显著不同，DA和LE显示出比SD高得多的PV+、CB+和CR+细胞数量，并且与其他两种菌株相比，DA中的CB+和CR+细胞数量特别高。这些研究结果表明，抑制系统可能是不同的开发属于不同的品系或显示不同的基础水平的活动和CaBP的表达，因此可能是不同的敏感rTMS协议的大鼠。
Using a rat model to study the cellular effects of repetitive transcranial magnetic stimulation (rTMS) with regard to changes in cortical excitability, we previously described opposite effects of continuous and intermittent theta-burst stimulation (cTBS, iTBS) on the expression of the calcium-binding proteins (CaBP) parvalbumin (PV), calbindin (CB) and calretinin (CR) in Dark Agouti rats (DA). While iTBS significantly reduced the number of cortical PV+ cells but did not affect the CB+ cells, cTBS resulted in a decrease in CB+ cells with no effects on PV+ cells. We concluded that activity of these classes of cortical interneurons is differently modulated by iTBS and cTBS. When testing two further rat strains, Sprague–Dawley (SD) and Long Evans (LE), we obtained deviating results. In SD, iTBS reduced PV and CB expression, while cTBS only reduced PV expression. In contrast, reanalysed DA showed reduced CB expression after cTBS and reduced PV expression after iTBS, while LE shows an intermediate reaction. CR expression was unaffected in any case. Interestingly, we found significantly different basal expression patterns of the CaBPs for the strains, with DA and LE showing much higher numbers of PV+, CB+ and CR+ cells than SD, and with particularly higher number of CB+ and CR+ cells in DA compared to the other two strains. These findings demonstrate that inhibitory systems may be either differently developed in rats belonging to diverse strains or show different basal levels of activity and CaBP expression and may therefore be differently sensitive to the rTMS protocols.
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影响因子：
7.7
作者：
L. Volz;A. Benali;A. Mix;U. Neubacher;K. Funke
通讯作者：
L. Volz;A. Benali;A. Mix;U. Neubacher;K. Funke
影响因子：
3.7
作者：
Gonchar, Y;Burkhalter, A
通讯作者：
Burkhalter, A
神经暴露长度、电流与神经夹角对神经兴奋阈值的影响
DOI：
10.1113/jphysiol.1927.sp002409
发表时间：
1927
期刊：
The Journal of Physiology
影响因子：
--
作者：
W. Rushton
通讯作者：
W. Rushton
影响因子：
3.7
作者：
Melchitzky, DS;Lewis, DA
通讯作者：
Lewis, DA
影响因子：
2.9
作者：
Chaudhury, Sraboni;Nag, Tapas Chandra;Wadhwa, Shashi
通讯作者：
Wadhwa, Shashi