Characterization of H7N9 influenza A viruses isolated from humans.
Characterization of H7N9 influenza A viruses isolated from humans.
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DOI：
10.1038/nature12392
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发表时间：
2013-09-26
期刊：
影响因子：
64.8
通讯作者：
Kawaoka, Yoshihiro
中科院分区：
文献类型：
--
作者：
Watanabe, Tokiko;Kiso, Maki;Fukuyama, Satoshi;Nakajima, Noriko;Imai, Masaki;Yamada, Shinya;Murakami, Shin;Yamayoshi, Seiya;Iwatsuki-Horimoto, Kiyoko;Sakoda, Yoshihiro;Takashita, Emi;McBride, Ryan;Noda, Takeshi;Hatta, Masato;Imai, Hirotaka;Zhao, Dongming;Kishida, Noriko;Shirakura, Masayuki;de Vries, Robert P.;Shichinohe, Shintaro;Okamatsu, Masatoshi;Tamura, Tomokazu;Tomita, Yuriko;Fujimoto, Naomi;Goto, Kazue;Katsura, Hiroaki;Kawakami, Eiryo;Ishikawa, Izumi;Watanabe, Shinji;Ito, Mutsumi;Sakai-Tagawa, Yuko;Sugita, Yukihiko;Uraki, Ryuta;Yamaji, Reina;Eisfeld, Amie J.;Zhong, Gongxun;Fan, Shufang;Ping, Jihui;Maher, Eileen A.;Hanson, Anthony;Uchida, Yuko;Saito, Takehiko;Ozawa, Makoto;Neumann, Gabriele;Kida, Hiroshi;Odagiri, Takato;Paulson, James C.;Hasegawa, Hideki;Tashiro, Masato;Kawaoka, Yoshihiro
禽流感A病毒很少感染人类，但如果它们感染并在人类之间传播，可能导致世界范围的爆发（大流行）。最近在中国发生的人类散发性感染H7N9亚型禽流感病毒（A（H7N9））引起了人们的关注，这是由于与这些感染相关的可观的病死率（>25%），潜在的人际传播情况，以及人类对该亚型病毒缺乏预先存在的免疫力。因此，在此，我们表征了两种早期人类A（H7N9）分离株，A/安徽/1/2013和A/上海/1/2013（H7N9;以下分别称为安徽/1和上海/1）。在小鼠中，安徽/1和上海/1比对照禽H7 N9病毒（A/duck/Gunma/466/2011; H7 N9; Dk/GM 466）和代表性大流行2009 H1 N1病毒（A/加州/04/2009; H1 N1; CA 04）更具致病性。安徽/1、上海/1和Dk/GM 466在雪貂的鼻甲中复制良好。在非人灵长类动物（NHP）中，Anhui/1和Dk/GM 466在上呼吸道和下呼吸道中有效复制，而常规人流感病毒的复制能力通常限于感染灵长类动物的上呼吸道。相比之下，Anhui/1在鼻内接种后在小型猪中没有良好的复制。最严重的是，安徽/1通过三对雪貂中的一对呼吸道飞沫传播。聚糖阵列表明，Anhui/1、Shanghai/1和A/Hangzhou/1/2013（本试验中检测的第三种人A（H7N9）病毒）与人病毒型受体结合，这一特性可能对病毒在雪貂中的传播性至关重要。Anhui/1对神经氨酸酶抑制剂的敏感性低于2009年H1N1大流行病毒，尽管两种病毒对实验性抗病毒聚合酶抑制剂同样敏感。在小鼠、雪貂和NHP中的强大复制能力以及安徽/1雪貂中的有限传播性表明A（H7N9）病毒具有大流行潜力。
Avian influenza A viruses rarely infect humans, but if they do and transmit among them, worldwide outbreaks (pandemics) can result. The recent sporadic infections of humans in China with a previously unrecognized avian influenza A virus of the H7N9 subtype (A(H7N9)) have caused concern due to the appreciable case fatality rate associated with these infections (>25%), potential instances of human-to-human transmission, and the lack of pre-existing immunity among humans to viruses of this subtype. Here, we therefore characterized two early human A(H7N9) isolates, A/Anhui/1/2013 and A/Shanghai/1/2013 (H7N9; hereafter referred to as Anhui/1 and Shanghai/1, respectively). In mice, Anhui/1 and Shanghai/1 were more pathogenic than a control avian H7N9 virus (A/duck/Gunma/466/2011; H7N9; Dk/GM466) and a representative pandemic 2009 H1N1 virus (A/California/04/2009; H1N1; CA04). Anhui/1, Shanghai/1, and Dk/GM466 replicated well in the nasal turbinates of ferrets. In nonhuman primates (NHPs), Anhui/1 and Dk/GM466 replicated efficiently in the upper and lower respiratory tracts, whereas the replicative ability of conventional human influenza viruses is typically restricted to the upper respiratory tract of infected primates. By contrast, Anhui/1 did not replicate well in miniature pigs upon intranasal inoculation. Most critically, Anhui/1 transmitted via respiratory droplets in one of three pairs of ferrets. Glycan arrays demonstrated that Anhui/1, Shanghai/1, and A/Hangzhou/1/2013 (a third human A(H7N9) virus tested in this assay) bind to human virus-type receptors, a property that may be critical for virus transmissibility in ferrets. Anhui/1 was less sensitive than a pandemic 2009 H1N1 virus to neuraminidase inhibitors, although both viruses were equally susceptible to an experimental antiviral polymerase inhibitor. The robust replicative ability in mice, ferrets, and NHPs and the limited transmissibility in ferrets of Anhui/1 suggest that A(H7N9) viruses have pandemic potential.
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人类感染新型禽源甲型流感 (H7N9) 病毒。
影响因子：
158.5
作者：
Gao, Rongbao;Cao, Bin;Shu, Yuelong
通讯作者：
Shu, Yuelong
影响因子：
3.7
作者：
Chen LM;Blixt O;Stevens J;Lipatov AS;Davis CT;Collins BE;Cox NJ;Paulson JC;Donis RO
通讯作者：
Donis RO
影响因子：
13.2
作者：
Liu, Xuhui;Li, Tao;Lu, Hongzhou
通讯作者：
Lu, Hongzhou
影响因子：
64.8
作者：
Imai, Masaki;Watanabe, Tokiko;Hatta, Masato;Das, Subash C.;Ozawa, Makoto;Shinya, Kyoko;Zhong, Gongxun;Hanson, Anthony;Katsura, Hiroaki;Watanabe, Shinji;Li, Chengjun;Kawakami, Eiryo;Yamada, Shinya;Kiso, Maki;Suzuki, Yasuo;Maher, Eileen A.;Neumann, Gabriele;Kawaoka, Yoshihiro
通讯作者：
Kawaoka, Yoshihiro
DOI：
10.1126/science.1213362
发表时间：
2012-06-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Herfst S;Schrauwen EJ;Linster M;Chutinimitkul S;de Wit E;Munster VJ;Sorrell EM;Bestebroer TM;Burke DF;Smith DJ;Rimmelzwaan GF;Osterhaus AD;Fouchier RA
通讯作者：
Fouchier RA